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The tumor is a serious threat to human life and health of common disease, radiotherapy is one of the main means of treatment, but due to lack of oxygen caused by the radiation resistance of tumor cells, radiation therapy often fail to achieve the desired effect. Therefore, to improve the hypoxic state of the tumor cells, increased radiosensitivity, radiotherapy and oncology research has become a hot spot. This study was to establish the murine Lewis lung carcinoma xenograft model, research Danshen injection and radiotherapy alone and in combination on mice xenografts Inhibition of HIF HIF-1alpha mRNA expression values ??of tumor tissue and blood VEGF concentration indicators, and to explore its mechanism of inhibitory effect on mice xenografts for clinical application Salvia improve tumor cells hypoxic state, increased radiosensitivity, to provide experimental basis to improve the efficacy of radiotherapy. Method: 1. Modeling: will return on behalf of 14-day source of Lewis lung tumors in mouse tumor tissue made of tumor cells were inoculated in 60 healthy female weighing 16-18g C57BL/6J mice right leg subcutaneous. 2 groups: 60 tumor-bearing mice were randomly divided into six groups, each group of 10. Namely: the model group, Salvia low-dose group (referred to as Dan low group), the Salvia high dose group (referred to as Dan high group), radiotherapy group, Salvia low-dose radiotherapy group (referred to Dan Low radiotherapy group), the Salvia high-dose radiotherapy group (referred to as Dan radiotherapy group). 3 dose and time: the tumor-bearing mice inoculated with the second day from the daily intraperitoneal injection of 0.2ml / day for 10 days. Radiotherapy group, Dan Low radiotherapy group, Dan high-radiotherapy group from the 7th day of the vaccination plus cobalt-60 irradiation local tumor 2Gy / day for 5 consecutive days. 4. Sampling and detection Indicators: Abstract 24 hours after the end of treatment the mouse eye blood test blood, the blood concentration of VEGF; stripping tumor measuring the weight of each group of tumor inhibitory rate was calculated; detection of tumor cells HIF-la mRNA expression value; were observed tumor microvessel density, apoptosis and organ metastases number. Results: (1) low group Dan, Dan, the high group, Dan low tumor weight of the radiotherapy group than the model group, the difference was statistically significant (P lt; 0.05), inhibition rates were 21.11%, 30.65%, 36.18% ; radiotherapy group, Dan high-radiotherapy group and model group trend to reduce the tumor weight, but the difference was not statistically significant (P GT; 0.05) inhibition rates were 1.01%, 21.61%; radiotherapy group group comparisons Dan Low radiotherapy group tumor lightest radiotherapy group, the difference was statistically significant (P lt; 0.01) Dan Low radiotherapy group and Dan high blood radiotherapy group of white blood cells, red blood cells, increased the hemoglobin number of the radiotherapy group, the difference was statistically significant (P lt; 0.05); Dan low-radiotherapy group and Dan high platelet count of the radiotherapy group decreased compared with the radiotherapy group, the difference was statistically significant (P lt; 0.05). Dan low group, Dan Low radiotherapy group, Dan high-radiotherapy group and model group comparison of HIF-1alpha mRNA expression values ??decreased, the difference was not statistically significant (P gt; 0.05); Dan low-radiotherapy group, Dan high radiation group and radiotherapy group of HIF-1alpha mRNA expression value lower, the difference was statistically significant (P lt; 0.01), and Dan Low radiotherapy group of HIF-1alpha mRNA expression reduced more significant. Dan the high group, radiotherapy group, Dan Low radiotherapy group, Dan high VEGF concentration of the radiotherapy group compared with the model group, the difference was statistically significant (P lt; 0.05); Dan Low radiotherapy group, Dan high-radiotherapy group and radiotherapy group Compare VEGF concentration decreased, the difference was not statistically significant (P gt; 0.05). 5 Dan the high group, radiotherapy group, Dan Low radiotherapy group and Dan high-radiotherapy group and the model group compared microvascular density (MVD) counts were reduced, the difference was statistically significant (P lt; 0.01); Dan the low radiotherapy group and Dan high radiotherapy with radiotherapy group microvessel density decrease, the difference was statistically significant (P lt; 0.01). Dan the high group, radiotherapy group, Dan Low radiotherapy group, Dan high apoptotic index of the radiotherapy group compared with the model group, the difference was statistically significant (P lt; 0.05); Dan Low radiotherapy group, Dan radiotherapy group radiotherapy group apoptosis index was significantly increased, the difference was statistically significant (P lt; 0.05). 7 light after the end of treatment were observed tumor histopathology, five tumor radiotherapy lung metastases, high-Dan Dan Low radiotherapy group, Dan high-radiotherapy group lungs see two tumor metastasis. Conclusion: Salvia injection combined with radiotherapy can reduce the value of HIF-1alpha mRNA expression and blood concentration of VEGF in the mouse xenograft to increase hypoxic cells oxygen pressure, reduce the number of hypoxic cells, inhibition of tumor angiogenesis, to improve the efficacy of radiotherapy, increasing the radiation on the tumor inhibition rate and induction of tumor cell apoptosis.
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