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Objective: To investigate the Tangshan region Han women cervical cancer risk factors, disease characteristics and pathogenesis, so as to explore the interleukin the -10 (interleukin-10, IL-10) gene polymorphism, human papilloma virus (human papillomavirus, HPV ) infection and cervical cancer among relations, for the prevention of cervical cancer and the HPV vaccine to provide the basis for the application and popularization in Tangshan. Methods: A retrospective epidemiological survey in the form of clinical epidemiology survey of 218 cases the Han hospitalized patients, including 103 cases of cervical cancer and 115 cases of normal cervical patients. The survey includes general information, past history, sexual history, menstrual and reproductive history, history of oral contraceptives, smoking history, marital status and education degree. Above influencing factors in the control group and the case group had no significant difference between the two groups were comparable. Venous blood 3ml, and specimens from about the size of 0.5cm × 0.2cm × 0.2cm cervical tissue. Using DNA extraction kit genomic DNA extracted from serum, phenol the imitation extraction method to extract the HPV-DNA in cervical tissue. Amplification refractory mutation system - polymerase chain reaction (ARMS-PCR) technology and polymerase chain reaction - restriction fragment length polymorphism (PCR-RELP) detection of the 218 patients with serum IL-10-1082G / A and-592A / C the presence and determine its genotype. PCR technology to detect the 218 patients in the cervical tissue HPV16/18 infection. Of IL-10-1082G / A,-592A / C and HPV16/18 of PCR amplification products were sequenced. Direct counting method haplotype construction and analysis of the IL-10 gene polymorphisms (SNPs). SPSS13.0 software for statistical analysis. Results: IL-10 gene polymorphism, cervical cancer and HPV infection susceptibility among relations results are as follows: the relationship between IL-10-1082G / A locus gene polymorphism with cervical cancer in cervical cancer susceptibility analysis of the IL-10-1082G / A genotype and allele frequencies: IL-10-1082G / A locus genotype and allele distribution frequency differences in the CC group and the control group had no significant sex (P gt; 0.05). The relationship between the 2 IL-10-592A / C locus gene polymorphism with cervical cancer in cervical cancer susceptibility and IL-10-592A / C locus genotype and allele frequency analysis shows: IL-10 distribution of-592A / C genotype frequencies in the CC group and the control group was not statistically significant (P gt; 0.05), the difference was statistically significant (P lt; 0.05) allele A frequency distribution. 3 IL-10-1082G / A locus linkage disequilibrium analysis of the IL-10-592A / C locus haplotype frequency distribution (GC, GA, AA) in the CC group were 6.8%, 18.0%, 75.2% in the control group were 8.7%, 27.0%, 64.3%, and the difference was significant (P lt; 0.05). 4 of HPV16, 18 infection and cervical cancer relationship: HPV16, 18 and the 1618 rate of infection in the case and control groups, the difference was significant (P lt; 0.05). Different pathological types HPV16, 18 and 1618, the infection rate is in turn 69.2% of cervical squamous cell carcinoma, 1.2% and 11.9%, 6.7% adenocarcinoma, and 46.7%, the adenosquamous carcinoma only HPV16 18 mixed infections one case. The different ages HPV16/18 the overall infection rate of pairwise comparisons no differences (P gt; 0.05); smoking of HPV16, HPV18, HPV16 18 infections affect not statistically significant (P gt; 0.05). 5 IL-10 gene promoter region-592A / C gene polymorphism distribution in HPV16, 18 tested positive and negative groups, The statistical analysis of the differences were significant, CA and AA genotype increased susceptibility to HPV18 infection ( PCA = 0.043, PAA = 0.035). 6 IL-10 gene promoter region-1082G / A gene polymorphism distribution HPV16, 18 tested positive and negative groups, the statistical analysis, the A allele distribution differences slightly with increased susceptibility to HPV16 infection (P = 0.049). Conclusion: 1 IL-10 gene polymorphism and CC susceptibility related to IL-10-592A / C locus allele A may be a genetic risk factor of cervical cancer, the occurrence of CC development, treatment has an important significance. 2 Tangshan region HPV16 and HPV16 18 mixed infection and cervical cancer is closely related main subtypes lead to squamous cell carcinoma, HPV16, HPV16, 18 mixed infections lead to adenocarcinoma, age and smoking of HPV16 and, 18 HPV16 18 mixed infections unrelated. 3 in Tangshan Han women IL-10 gene promoter region-592A / C gene polymorphism may be with HPV16 and 18 infections lead to cervical cancer are relevant. -1082G / A locus A allele was associated with HPV16 infection causes cervical cancer.
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