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Objective: Chronic kidney disease (chronic kidney disease, CKD) patients with presence of oxidative stress status in vivo has been confirmed that the changes influencing factors for oxidative stress, as well as between the different CKD stages, however, is unclear. Arachidonic acid (arachidonic acid, AA) and tumor necrosis factor-α (tumor necrosis factor-α, TNF-α) are two important inflammatory factors, and the complex relationship between oxidative stress. Malondialdehyde (malondialdehyde, MDA) is one of the important markers of oxidative stress. This article by AA, TNF-α and MDA to explore the impact of oxidative stress in patients with CKD factors as well as the different CKD stages between oxidative stress changes. Methods: The study of 105 cases, including: non-diabetic primary glomerular disease diagnosed by clinical and renal biopsy check the 79 patients (55 males and 24 females; which CKD1, 2 of 29 cases, CKD3 4 of 21 cases, CKD5 of 29 cases, 10 cases which CKD5 of patients receiving continuous ambulatory peritoneal dialysis (continuous ambulatory peritoneal dialysis, CAPD) treatment of CAPD dialysis treatment 3 months after self-control); ② 12 cases diagnosed by clinical and renal biopsy examination diabetic nephropathy CKD5 period non-dialysis patients (7 men, 5 women); ③ The normal control group of 14 patients (7 men, 7 females). Each group observed object morning fasting blood 3ml, collected in the disposable test tubes, placed on the stationary two hours to room temperature, the serum was separated, loaded EP tube, and stored at -70 degrees in the refrigerator, with the ELISA kit ( ELISA) measured serum free AA, TNF-α, MDA, high-sensitivity C-reactive protein (high sensitive C-reactive protein, hs-CRP) concentration. Results: ① primary glomerular disease: CKD1 period and CKD3 MDA levels of the patient's body without significant difference (P gt; 0.05), but were significantly higher than CKD5 of (P lt; 0.05) ; AA and MDA levels were negatively correlated with the antioxidant effect of TNF-α and MDA levels were positively correlated with the pro-oxidation; hs-CRP levels of each of the patient's body is no significant difference. ② the diabetic nephropathy CKD5 period MDA level of the patient's body and hs-CRP levels were significantly higher than the primary the glomerular diseases CKD5 patients, AA with the promotion of the role of oxidative stress in diabetic nephropathy. Before to ③ the primary glomerular diseases CKD5 in period CAPD treatment, after the patient's body hs-CRP and MDA levels no significant differences. Conclusion: ① primary glomerular disease: oxidative stress along with decreased kidney function decline; patients the body's level of inflammation is basically the same, except infection. ② the diabetic nephropathy CKD5 period of the patient's body oxidative stress and inflammation levels than primary glomerular disease CKD5 of significantly enhanced. Oxidative stress in patients (3) short-term peritoneal dialysis primary glomerular disease CKD5 period, the level of inflammation is not a significant impact. ④ TNF-α promote oxidative stress of primary glomerular disease in patients with CKD CKD etiology; AA is not the same role for oxidative stress.
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