Dissertation > Excellent graduate degree dissertation topics show

The Gene Polymorphism and Serum Content of TNF-α in Restenosis Patients after PCI

Author: ZhengJunYi
Tutor: LiuYin
School: Tianjin Medical University
Course: Internal Medicine
Keywords: percutaneous coronary intervention(PCI) tumor necrosis factor-α(TNF-α) gene polymorphism restenosis
CLC: R541.4
Type: Master's thesis
Year: 2011
Downloads: 10
Quote: 0
Read: Download Dissertation

Abstract


Objective:To find the risk factors of restenosis after PCI and reveal the relationship with gene polymorphism and serum content of tumor necrosis factor-α(TNF-α).Methods:360 patients were selected in our study with reexamination PCI since October 2005 to May 2007 to investigate the relationship of the basic data for PCI operative and restenosis. On the basis of the review of coronary angiography results, patients were divided into two groups, restenosis group(n=134) and control (non-restenosis) group(n=226). On the other hand, each patient’s genome DNA was extracted from blood to amplify the unique fragment of TNF-α-308G/A site by using method of PCR with specific primers. The amplicons were identified by restriction analysis with StyⅠenzyme. According to the agarose gel electrophoresis results, TNF-a genotype was invided into three types, GA(n=324),GG(n=36), AA(n=0). Serum TNF-αcontent was also quantified using ELISA method.Results:1. Using the logistic regression analysis, we found men (p=0.024), high weight (p=0.018), high lipoprotein (a) (p=0.007), target lesion site of right coronary artery (p=0.004), small vessel (diameter<3mm) (p=0.017), were significant difference in control and restenosis groups. Person has factors above was tend to restenosis after PCI.2. TNF-αgene polymorphism has GG, GA, and AA types. In this study, GAtype was 36 of 360 patients(10%), GG type was 324 of 360 patients(90%), AA type wasn’t find. The GG and GA type were 119 (88.8%) and 15 (11.2%) respectively in ISR group, compared with 205 (90.7%) and 21 (9.3%) in NISR group, p=0.588,χ2=0.338。The allele frequency of A were 5.6% in ISR group and 4.6% in NISR group, meanwhile the G were 94.4% and 95.4% in two groups, p=0.598,χ2=0.32.3. The serum TNF-αcontent was 46.44±5.66pg/ml in ISR group compared with 45.83±3.65pg/ml in NISR group, p=0.797; which was 46.59±5.21pg/ml and 43.86±2.87pg/ml respectively in GA type and GG type, p=0.071. Conclusion:1. The high-risk patients can be marked by factors such as men, high weight,high lipoprotein (a), target lesion site of right coronary artery, small vessels with diameter<3mm, who need close follow-up and intensive drug therapy.2. Through the statistical analysis of polymorphism of TNF-αpromoter-308G/A site of 360 patients, we found gene polymorphism distribution of TNF-a promoter-308G/A site is consistent with the distribution of Han people but different from foreign investigation, which hinted the gene polymorphism distribution of TNF-αpromoter-308 G/A site maybe has racial differences. Meanwhile the statistical results revealed that there is no relationship between gene polymorphism of TNF-αpromoter-308G/A site and restenosis after PCI, which is similar with foreign report. There has no same investigation domestically.3. Through the statistical analysis of serum TNF-αcontent of 360 patients, we found there is no statistically significant difference in ISR and NISR groups. Thus, there is no relationship between serum TNF-αcontent and restenosis after PCI. The serum TNF-αcontent of GA type patients is higher than GG type patients, but there is no statistically difference neither. The results hinted there is no relationship between serum TNF-a content and polymorphism of TNF-a promoter-308G/A site.

Related Dissertations

  1. CYP2C19 Gene Polymorphism in Patients with Ischemic Cerebrovascular,R743
  2. The Relationship of Polymorphisms of Methionine Synthase Reductase(MTRR) Gene and Plasma Homocysteine Levels with Myocardial Infarction,R542.22
  3. Effects of Receptor Activity Modifying Protein-1 Transplantation of Mesenchymal Stem Cells on Postangioplasty Restenosis and Cardiac Function after Myocardial Infarction in Rabbits,R542.22
  4. Association of Interleukin-6 Gene Polymorphisms with Susceptibility to Coronary Heart Disease in Asian Populations: a Meta-analysis,R541.4
  5. The Myocardial Protection Effect of High-Dose Atorvastatin Pretreatment Before Percutaneous Coronary Intervention in Acute Coronary Syndrome,R541.4
  6. Study of the Expression of Toll-like Receptor 2, 4mRNA and Polymorphisms of Patients with Leprosy,R755
  7. MDR1 and CYP3A gene polymorphisms on digoxin plasma concentrations of,R96
  8. SLC2A9 gene eighth exon 137A / G polymorphism and diabetic patients with hyperuricemia relationship,R587.1
  9. The Expression of DNA Adducts dA in Chronic Atrophis Gastritis, Precancerous Lesions and Gastric Cancer and the Relationship with the CYP2E1 Gene Polymorphism,R735.2
  10. The Influence of Innate Immune Molecules and Cells in the Pathogenesis of Autoimmune Disease in Central Nervous System,R392
  11. Application of SYBR Green I Real-time PCR in ABO Genotyping,R440
  12. PEF AT2R mediated gene expression in the vascular localized impact on neointimal,R541.4
  13. The Correlation Research between the Gene Polymorphisms of Five CYP450 Isoforms of Enzymes and the Differences of Metabolic Ratio of Cocktail Probes,R969.1
  14. The Polymorphism of Sites 373 and 1688 of PECAM-1 Gene in Mucocutaneous Lymph Node Syndrome in Children,R758.6
  15. Association between Genetic Polymorphisms of the FP Receptor (Prostaglandin F2α Receptor) Gene and Response to Latanoprost,R775
  16. CHD patients with depression nitric oxide synthase gene G894T polymorphism,R749.4
  17. The Analysis of the Polymorphism of the Plasminogen Activator Urokinase (PLAU) in Alzheimer’s Disease (AD) of Uygurs and Hans in Xinjiang,R749.16
  18. Analysis of the CYP2C19 Genetic Polymorphism in Uigur and the Association with Effects of Escitalopram,R749.41
  19. The Correlation Research of UGT Genetic Polymorphism and Valproic Acid’s Serum Concentration,R742.1
  20. The Polymorphism of Parkin Gene in Uygur and Han Patients with Parkinson’s Disease in Xinjiang,R742.5
  21. Xinjiang Uygurs and Hans Phosphodiesterase 4D Gene SNP83 Polymorphism Analysis of Ischemic Cerebrovascular Disease,R743.3

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Coronary arteries ( atherosclerosis ),heart disease (CHD)
© 2012 www.DissertationTopic.Net  Mobile