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[Objective] explore the protective effect of the tested drugs bud Ye Xuelian water extract of mouse blood system, immune system, reproductive system, etc.. [Research] to radiation injury in mice as a research object, given different doses of bud Ye Xuelian water extract orally, 14 days after to accept 4Gy the X-ray disposable body irradiation, and continue after gavage, respectively, at different points in time mice was detected by the indicators. 1. Micronucleus test: the first day after irradiation, the mice were sacrificed to measure the rate of micronucleus. Blood system: 1,3,6,9,12 days after irradiation, the mice were killed, detection of venous blood in the RBC, WBC, PLT, Hb content; the days 3,6,9 detection of mouse bone marrow after irradiation. number of nucleated cells, bone marrow DNA content (A value). The immune system: the 1,3,6,9 days after irradiation to measure the body weight of mice, spleen and thymus weight and spleen and thymus index were calculated. Reproductive system: 35 days after irradiation the mice were killed, measuring mouse sperm malformation rate. [Findings] 1. The micronucleus experimental results show that the model group, the number of micronuclei, each administered radiation group were lower than the positive group (cyclophosphamide group), which, the middle dose group and high dose group was significantly lower than the positive group, The existence of significant statistical difference, P lt; 0.01 blood system results show that, compared with the irradiated group, each administration group of mice irradiated WBC, PLT were significantly elevated, the presence of significant statistical differences, P lt; 0.01. Compared with the irradiated group, the administration group of mice irradiated RBC, Hb content, bone marrow nucleated cell count, bone marrow DNA content (A value) values ??are slightly elevated, but no statistically significant difference, P gt; 0.05. immune organ weight and organ index measurement results show that the irradiation group, administration irradiation group mice spleen weight index were higher than the irradiated group, high dose group than in the irradiated group, there is a statistically learning differences, P lt; 0.05. Thymus weight index of each administration group of mice irradiated and irradiated groups compared to slightly higher, and there is a significant difference, P lt; 0.05 mouse sperm malformation rate results show that the administration irradiation group compared with the irradiated group, low dose, medium dose, high-dose mice sperm abnormality was less than the radiation group. Among them, the middle dose group and high dose group was significantly lower than the radiation group, there was significant difference, P lt; 0.01. [Conclusion] the bud Ye Xuelian water extract of the active ingredient of the mice's blood system, immune system, reproductive system, there is a certain degree of radiation injury. Blood system of mice WBC, PLT impact significantly; RBC, Hb content, bone marrow nucleated cell count, bone marrow DNA content (A value) was not obvious. Immune system of mice spleen, thymus affects both, suggesting cell and humoral immune trends. 3 sperm malformation rate experiments prompted the germ cells of mice also have some degree of radiation injury. 4 mouse micronucleus test, model group, the number of micronuclei, each administered radiation group were lower than the positive group (cyclophosphamide group), which, the middle dose group and high dose group was significantly lower than the positive group, suggesting that for small mouse chromosomes and spindle fibers have some protective effect.
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