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New materials modified electrode and its application in electrochemical sensors

Author: ZhangYao
Tutor: JiaNengQin
School: Shanghai Normal University
Course: Analytical Chemistry
Keywords: Electrochemical sensors Redox proteins Nanomaterials Direct electrochemistry Bioelectrocatalysis
CLC: TP212.2
Type: Master's thesis
Year: 2011
Downloads: 179
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Abstract


Electrochemical biosensor has good selectivity, high sensitivity, fast analysis, testing, low cost, equipment required is simple and can be online in a complex system of continuous monitoring, easy to realize the advantages of miniaturization in the chemical, biomedical, environmental monitoring , food, medicine and military fields has important application value. The novel nanomaterials and a polymer material applied to the preparation of biosensors can significantly improve the performance of the sensor. This thesis were used graphene oxide (GO), ordered mesoporous carbon and silicon nanocomposites (MCSi) and polylactide-co-glycolide (PLGA) for the construction of bio-sensing interfaces. Specific studies are as follows: (a) polymerization Prussian Blue by electroporation into graphene oxide modified glassy carbon electrode, forming a novel graphene oxide / Prussian blue composite film by Raman spectroscopy of the composite film successful modification. Graphene oxide electrochemical experiments show greatly improved electrochemical response of Prussian blue. The composite film modified electrode for detecting hydrogen peroxide, hydrogen peroxide sensor prepared in its linear range 5.0 × 10-6-1.2 × 10-3M, low detection limit 1.22 × 10-7M (S / N = 3) high sensitivity 408.7uA · mM-1 · cm-2 and reproducible. Glucose oxidase by chitosan is fixed to the composite film made of a glucose sensor. By Amperometric Glucose Biosensor characterization that there is a good catalyst for glucose response, wide linear range 0.1-13.5 mM, low detection limit 3.43 × 10-7M (S / N = 3), high selectivity, and there is a good weight now and stability. In the actual detection of human serum sample recovery, indicating that the sensor is used to detect glucose in real samples. Thus, graphene oxide / Prussian blue composite film modified electrode in an electrochemical detection and possible biological detection has good application prospects. (2) use of ordered mesoporous carbon and silicon nanocomposites (MCSi) fixed myoglobin (Mb) was prepared Mb-MCSi film modified electrode was studied myoglobin in Mb-MCSi membrane and electrode direct electron transfer between the surface and electrocatalytic behavior. X-ray diffraction (XRD), transmission electron microscopy (TEM), N2 adsorption-desorption curves characterize ordered mesoporous carbon and silicon composite material. Electrochemical impedance spectroscopy (EIS) showed that myoglobin MCSi firmly fixed to the composite material. Ultraviolet spectra (UV) results showed MCSi Mb is fixed to the composite material, the hemoglobin has not changed the surrounding microenvironment, myoglobin maintain native conformation and biological activity. Meanwhile, Mb-MCSi modified electrode for H2O2 catalytic significant power in the range of 1.0-20.0uM its catalytic peak current size and concentration of H2O2 has a good linear relationship. (3) using a biodegradable polymeric material polylactide-co-glycolide (PLGA) and ionic liquids (ILs) composite membrane protein as a substrate to the embedding, and studied its bioelectrical chemical properties. Select hemoglobin (Hb) as a model protein research PLGA composite membrane systems and ionic liquids. Discussion of the sweep speed, pH, and other interfering substances influence the enzyme biosensor, the experimental results show that the sensor detecting hydrogen peroxide (H2O2) detection limit of 2.37 × 10-7 M (S / N = 3), the linear range of 5.0 × 10-6-8.05 × 10-3 M, at this concentration of H2O2 in the range of current and its catalytic reduction proportional to the concentration. Electrochemical biosensors prepared response, high sensitivity, high selectivity and good reproducibility.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Chemical sensors
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