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Cashmere whole life history stages microscopic fungus bubble and ultrastructural studies

Author: WangXiaoLi
Tutor: WangXiaoLi
School: Jilin Agricultural University
Course: Crop Genetics and Breeding
Keywords: Full Cashmere bubble bacteria Phenotype plasmodia Life history Microstructure Ultrastructure
CLC: Q93
Type: Master's thesis
Year: 2011
Downloads: 25
Quote: 0
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Abstract

This experiment used the material for the project Physarum bacteria Physarum species Physarum Trichocomataceae full Cashmere bubble bacteria (Physarum mellrum). Full Cashmere bubble fungus is a typical phenotype plasmodia, can continue to deform and flow, crawling on the surface of the base material of food intake, but also the same as the fungal spores into the environment through the bad. Full Cashmere bubble bacteria in 2% agar medium, fed sterile water and oat flour, at a temperature above 20 ℃ grew well. Full Cashmere bubble bacteria can easily be contaminated by fungi and nematodes, the turntable culture through purification of contaminants. Full Cashmere bubble bacteria difficult to form sclerotia, but it can form fruiting bodies. Through the different periods of plasmodia and fruiting phased drawn, usually drawn once every 15 minutes to observe the changes in the internal structure; while the mature spore germination, gradually observed at all stages microstructure protoplasm and ultrastructural changes . The results showed that: all cashmere large and thick foam bacteria with a fan-shaped mesh structure, the edge of a clear, are bonded semi-fluid. Through experiments in vivo staining and photomicrography other methods, all derived strains Cashmere bubble inclusions are mainly calcareous and free nuclei; bacteria compared with rhythmic pulse stream and from the protoplasm. Full induced by drying and hunger is difficult to form sclerotia Cashmere bubble bacteria, plasmodia by polycondensation dried lethal: however, all very easy Cashmere bubble bacteria induce the formation of fruiting bodies. In hunger and light and non-light both cases, the quality of the original group were formed fruiting bodies, there is no clear distinction. Full Cashmere bubble bacteria with white handle, fruiting bodies of a gradual transition in color from white to black, and finally turned gray. Cyst two ways: one is to separate the cysts one, the other one is connected twenty-three cysts a joint capsule. Spores by scanning electron microscope, it can be seen all bacterial spores cashmere bubble surface warts smooth inner plasma membrane, spore spores between wire and has a special structure uncertain. Will be taken from different periods plasmodia and fruiting in FAA, the application of technology and a variety of paraffin sections stained cells were observed microstructural changes. The results observed from plasmodia of polycondensation to form fruiting bodies is the gradual formation of spores and cysts, are also included within the spore formation of filaments and inclusions. The mature spores placed in the concave slide, dropping nutrient solution, spores began to germinate after 2-3 days. The observation: Germination is divided into two kinds: \The former circular protoplasm, the spore wall split \Motile cells of the kidney-shaped, with a flagellum; sticky deformable body may extend pseudopodia feeding. With the concave slide in the amount of water gradually from motile cells into a sticky deformable body. Two swimming cells or sticky deformable body combine to form the zygote, formed after the young plasmodia, to continue to foster become phenotype plasmodia. Will be part of the concave slide flame dried, the magenta dye solution can be observed in vivo protoplasm and nucleus deformation sticky. This experiment also includes acridine orange staining. Fresh plasmodia, cytoplasmic actin cytoskeleton and bacteria veins after by acridine orange staining was observed under a fluorescence microscope, can be clearly observed in the nucleus and green fluorescent bacteria veins and red fluorescent cytoplasmic actin skeleton. This article from the full life cycle of bacteria Cashmere bubble departure, the application of stereo microscope, optical microscopy, scanning electron microscopy and fluorescence microscopy to observe the bacteria throughout the whole life cycle of Cashmere foam cells in various stages of microstructure and ultrastructure, in order for the slime molds cell biology to provide some evidence.

Cashmere whole life history stages microscopic fungus bubble and ultrastructural studies

Abstract

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