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Preparation of Diacylglycerol from Esterification of Fatty Acid Catalyzed by Phospholipase A1

Author: WangLiLi
Tutor: HuChangYing；WangYong
School: Jinan University
Course: Cereals, Oils and Vegetable Protein Engineering
Keywords: Diglyceride Phospholipase A1 Esterification reaction High temperature gas chromatography Molecular distillation
CLC: TQ463.2
Type: Master's thesis
Year: 2011
Downloads: 56
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Abstract


Diacylglycerol (DAG) is an important functional oils, having inhibit obesity, reducing postprandial blood glucose , and other functions . This paper as raw material to soybean oil fatty acid (FFA) and glycerol (Gly) , phospholipase A1 (Lecitase Ultra) catalyzed esterification reaction of the DAG . High temperature gas chromatography method for analysis the grease samples of FFA and glycerides , and through a molecular distillation purification of crude the DAG , DAG product of high purity . Single factor and response surface experiments to optimize the reaction conditions . The reaction time (2-10 h), add water (2-14 wt-% of substrate ) , plus the amount of enzyme (10-120 U / g of substrate ) , and the reaction temperature (30-70 ° C ) and substrate the molar ratio of Gly : FFA ( 0.5-2.5 ) esterification rate . The optimal reaction conditions are as follows: reaction temperature 40 . C , the amount of water added 8 wt-%, the reaction time of 8 h , substrate molar ratio (Gly: FFA) 2.2:1, enzyme dosage of 90 U / g . In this reaction , the fatty acid esterification rate was 81.12% , the DAG content of 37.62% . Establish a high-temperature gas chromatography method for analysis the grease samples of FFA and glycerides , optimized , glyceryl total analysis time is shortened to less than 10 min . Retention time qualitative and quantified by external standard , qualitative and quantitative accuracy . The FFA, Monoglycerides ester ( MAG ) , DAG, the TAG gas chromatography analysis of the standard curve , in the concentration range of 0.5 to 20.0 mg / mL , a good linear relationship between peak area and analyte concentration . Recovery test showed that the average recoveries of 95.69-105.78 % , RSD between 0.27 to 2.98% . The esterification mixture after standing stratified get contain DAG reservoir . Using molecular distillation to remove unreacted FFA and byproducts in the reservoir and the MAG , 170 . C distillation column temperature the DAG product purity of 83.1% , the yield was 42.7% . Simultaneous determination of the the molecular distillation heavy phase and light phase DAG content and fatty acid composition .

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