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Structural Changes of Intramuscular Connective Tissue during Growth of Wuzhumuqin Sheep

Author: SuYaLa
Tutor: GeRiLeTu
School: Inner Mongolia Agricultural University
Course: Agricultural Products Processing and Storage
Keywords: Endomysium Perimysium Structural changes in Type I collagen Type III collagen
CLC: S826
Type: Master's thesis
Year: 2011
Downloads: 22
Quote: 1
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Abstract


Thesis excellent place the varieties ujimqin sheep ( 1-18 months old) longissimus muscle sampling points , using histological staining observed cross-section diagram of the muscle tissue , determination of intramuscular using OPTPRO2008 software the thickness of the film and perimysium ANOVA analysis using SAS software With the increase in the month-old endomysium and perimysium thickness variation ; muscle fibers to remove cell digestion , followed by the use of the scanning electron microscope endomysium the perimysium structure analysis with the increase in the month-old endomysium perimysium structure of variation ; ⅰ and Ⅲ collagen extracted test by SDS-PAGE electrophoresis identification ; Coomassie ANOVA analysis blue method and do the standard curve method ⅰ and Ⅲ intramuscular connective tissue collagen content was measured using SAS software to increase as the month intramuscular connective tissue type Ⅰ and Ⅲ collagen content . The results show that : (1 ) increase as the month endomysium thickness gradually increased . Age of 6 months and 9 months of age , between the age of 12 months and 18 months of age , the difference was not significant (P gt; 0.05), compared with the 1 -month-old between the old and the differences were significant ( P lt; 0.05 ) . Increase as the month , the thickness of the first six months of the perimysium significant increase ( P lt; 0.05 ) , but then decreased significantly ( P lt; 0.05 ) . (2) overall , endomysial rendered honeycomb structure, endomysial collagen fiber is constituted by the mesh-like structure ; perimysium presents several layers of membrane structures, perimysium collagen fiber is constituted by the wavy structure . With the increase in ujimqin sheep -month-old , the aperture of the endomysium the cellular gradually increased , the network structure of the collagen fibers of the endomysium more closely perimysium collagen fibers wavy structure becomes more rules . ( 3 ) intramuscular connective tissue type Ⅰ collagen and type III collagen protein content increased significantly ( P <0.05 ) increase as the month .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Sheep
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