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The straw mushroom biological efficiency of approximately 20%, significantly lower than other edible fungi, and bacteria is one of the important means of production in the straw mushroom production, straw mushroom spawn quality is directly related to the yield and quality of the fruiting . In this study, 1 screen excellent, V0023, V the Youxi three straw mushroom strains for the material, from the three strains produced strains purity, bacteria vitality, initially established a set of straw mushroom spawn quality control technology. The main findings are as follows: 1 strains produced three strains of different straw mushroom cultivation kinds of recipes, moisture content, pH, incubation temperature preservation time of fruiting yield, the results showed that: cotton seed hulls 85%, wheat bran 10%, superphosphate 1%, 4% lime recipe yields the highest water content of 62%; straw mushroom spawn most suitable moisture content of 59% -62%; straw mushroom strains best suited to add 4 % -6% lime; incubation temperature of 21 ℃ -24 ℃ straw mushroom spawn growth slow to 27 ° C to -36 ° C bacteria grow quickly, but 21 ℃ - 36 ℃ culture the straw mushroom species fruiting yield differences ; increases with the time of Culture Collection, mushroom production is in serious decline. The 2 strains purity to three straw mushroom strains as materials, respectively, from the three aspects of the bacteria, viruses, fungi and detected. Bacteria detected in the the straw mushroom normal strains bottle inoculated with different concentrations of the bacteria, so that the formation of a recessive pollution, then flat crossed recessive pollution detection and three methods of liquid culture method and the boil-PCR method sensitivity detection, the results showed that: The three methods can be used for the straw mushroom strains recessive bacterial contamination detection; streak plate method and boiling-PCR method detection limit concentration of 10 to 4 / g bacteria; liquid culture assay the limit of concentration of 10 to 3 / g bacteria. DsRNA technology of virus detection using extraction and enzymatic method validation, the results show that: the positive control can detect dsRNA, three straw mushrooms by the enzymatic no bands, therefore, three straw mushroom strains were not infected with the virus, dsRNA method can be used to detect whether the straw mushroom spawn infected. The mold detection of the plate method can be used, the detection limit of this method concentrations of 100 spores per gram of bacteria. 3 strains vitality through the straw mushroom spawn preparation of different preservation time determined by several strains of aging-related indicators, the results show that: the germination rate of the bacteria, cellulase activity, polyphenol oxidase activity, malondialdehyde content of the nuclei of the proportion of nucleated cells, as well as strains of total RNA extracted results with preservation time and yield was significant, can be used as indicators reflect straw mushroom spawn vitality; amylase activity, protease activity, xylanase activity of pH value, acid phosphatase activity, peroxidase activity and superoxide dismutase activity preserved time and yield no significant correlation, can not as a reflection of the vitality of straw mushroom spawn indicators.
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