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The Fujian tea germplasm rich 30 parts of Fujian oolong tea varieties (lines) using RAPD markers to analyze genetic diversity and genetic relationship among varieties. In vitro preservation Golden Phoenix for the material, oolong tea germplasm restrictive vitro conservation methods. The main findings are as follows: 1. Tea plant are rich in polyphenols, polysaccharides and other impurities, increase the difficulty of extracting high quality DNA. This study on the basis of previous studies, worked out a set of simple, rapid extraction of the tea plant DNA modified CTAB method. The results show that, Tea Tree DNA extracted in both quality and quantity to meet the requirements of the RAPD-PCR. 2 from 37 RAPD random primers screened 11 polymorphic primers, RAPD-PCR amplification of 30 tea varieties (lines), total of 125 bands were amplified polymorphic bands of 108 Article polymorphism percentage was 86.40%. Application NTsys 2.10e software, genetic similarity coefficients, 30 varieties of tea between genetic similarity coefficient between 0.41 0 .83. The results show that the wide oolong tea germplasm genetic variation, polymorphism and genetic resources. Based on the RAPD results for the test of tea varieties cluster analysis, construct a dendrogram revealed the genetic relationship between the different tea varieties and genetic similarity. Similarity coefficient was 0.40 divided 30 tea resources are divided into three groups, including Shau Yee varieties separately as a class, most originating in a large class of Anxi tea varieties (lines) poly. The female parent of the Goddess of Mercy Iron Goddess of Mercy, RAPD analysis to get both Nei genetic similarity coefficient is 0.82, and the Goddess of Mercy Tieguanyin kinship closer, verify the reliability of the identification of RAPD technology as a variety kinship. RAPD analysis, to lay a theoretical basis for future breeding work to improve breeding efficiency. 4 in oolong tea germplasm preservation in vitro studies, the varieties of Golden Phoenix, comparing different concentrations of mannitol, sucrose penetrant and PP 333 and ABA growth inhibitors restrictive factors of tea plantlets in vitro conservation. The results showed that, in 15 g / L mannitol treatment added to the 1/2MS medium, save the best, the best medium for 1/2MS 15 g / L mannitol, seven months after each treatment survival this medium, the highest, 82.2%. The basic medium suitable for the Golden Phoenix long-term from the body subculture 1/2MS, the optimal sucrose, PP 333 and ABA concentration: 30 g / L, 20 mg / L and 0.5 mg / L.
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