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Objective: To vitro hippocampal slices as experimental subjects , using extracellular recording techniques , initial observation of propofol on vitamin A deficiency in hippocampal long-term potentiation potentials . Methods: randomly selected normal and vitamin A deficiency SD rats were sacrificed and prepared hippocampal slices , in a mixed gas saturated artificial cerebrospinal fluid were incubated for 2 hours and choose better brain slices into the experiment , brain slices grouping and processing factors are as follows : N1 group : normal control group , no vitamin A deficiency , without giving propofol treatment ; N2 group : None of vitamin A deficiency , brain slices perfusate by adding propofol to a final concentration of 50μmol / L; VAD1 group: vitamin A deficiency model , without giving treatment with propofol ; VAD2 group : Vitamin A deficiency model of brain slices perfusate was added propofol to a final concentration of 50μmol / L. The experimental process , first to single channel pulse stimulation ( Test stimulation , the wave width 0.2ms) evoked Qunfeng potentials (population spike, PS), intervals 5 min recorded once PS amplitude of the , take 3 times a average value as baseline value , record in each group at baseline . Then N2 group and VAD2 group perfusate replaced with a final concentration of 50μmol / L propofol artificial cerebrospinal fluid ( flow rate of about 1ml/min), continue to give single- pulse stimulation ( stimulation intensity and pulse width with the test stimulus ) , each 10min once observed evoked potentials 1h. Then mining fresh artificial cerebrospinal fluid (aCSF) pairs of propofol adequately elution , elution given after 100 Hz 's high-frequency tetanic stimulation ( stimulus intensity and wave width same test stimulation ) , static interest-bearing 10min, and then given test stimulation, every 10 min once , the observation 1 h. N1 group and VAD1 group was not propofol treatment , and the remaining experimental operation and observation records and N2 group , VAD2 same set , given tetanic stimulation , the same observation , recording PS activity 1h. The results I after HFS , with N1 group , N2 group LTP relative amplitude was significantly inhibited , a significant reduction ; and VAD1 group , VAD2 group LTP relative amplitude was significantly inhibited , a significant reduction ; and N2 group , VAD2 group LTP relative rate of decline is more obvious , VAD2 group tetanic after stimulation , and its PS electric potential basic remained at basal level . Conclusion Propofol significantly inhibited rat hippocampal slices LTP, and vitamin A deficiency in rats inhibitory effect stronger .
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