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Objective back the small incision organizations block plantation build rabbit VX2 renal carcinoma model, the evaluation back small incision organizations block plantation build the security and reliability of rabbit VX2 renal carcinoma model, detected after radiofrequency ablation of peripheral blood cytokine concentration to explore radiofrequency ablation of kidney tumors caused by changes in the immune system and mechanisms. 20 New Zealand white rabbits, using the back of a small incision organizations block plantation build rabbit VX2 renal cell carcinoma model, two weeks after the rabbits were sacrificed, necropsy and histopathological examination, clear rabbit the back small incision organizations block plantation build rabbit VX2 the success rate of kidney cancer model, tumor growth was observed situation and the presence of adjacent organs, lymph node metastasis. 30 New Zealand white rabbits were randomly divided into three groups, namely radio frequency treatment group, surgical treatment group, tumor control group. Construct renal VX2 tumor model, radio frequency treatment group, the surgical treatment group were radiofrequency ablation, radical nephrectomy in treating kidney tumors, tumor control group only received the control treatment, respectively, after 7 days, 14 days, 28 detected on day three groups of rabbits in the peripheral blood of IFN-gamma, TNF-alpha and interleukin-2 ,6,10 concentration observed after radiofrequency ablation of rabbits immune system, underwent CT imaging to observe RF before and after ablation imaging changes. Three groups of rabbits after 7 days, 14 days in each group were sacrificed 2 observed postoperative pathological changes. 28 days after treatment, the remaining three groups of rabbits were sacrificed for pathological examination, evaluation of effectiveness of radiofrequency ablation. 20 New Zealand white rabbits renal VX2 tumor model were successfully constructed, all tumors were single, was exogenous growth, regular shape; no tumor to break through the renal capsule, more in line with the early clinical kidney cancer characteristics. RF treatment group of 10 rabbits RF ablation, pathological examination showed no obvious residual tumor, CT scan showed the tumor tissue damage, enhanced scan no enhancement. RF treatment group and after surgical treatment group compared with 7 days: peripheral blood IFN-gamma concentration, and IL-2, IL-6, TNF-alpha and IL-10 concentration was no significant difference; after 14 days : IL-6 concentration in the peripheral blood is reduced, while IFN-gamma concentration, IL-2, no significant difference in the concentration of TNF-alpha and IL-10; 28 days after comparison: in the peripheral blood of IL-2 and IFN The-gamma rise lower concentration of IL-6 and IL-10, TNF-alpha concentration was no significant difference. RF treatment group the same tumor control group were compared with seven days: in the peripheral blood reduced IL-6 and TNF-alpha, IFN-gamma increased, while IL-2, IL-10 concentration was no significant difference; after 14 days : elevated in peripheral blood of IL-2 and IFN-gamma, IL-6 and IL-10 decreased, while no significant difference in the TNF-alpha concentration; 28 days after compare: peripheral blood of IL-2 and IFN-gamma concentration, lower concentration of IL-6 and IL-10, TNF-alpha concentration was no significant difference. Surgical treatment group the same tumor control group were compared with seven days: no significant difference in IFN-γ, IL-10 and IL-2 concentration in the peripheral blood, and TNF-alpha and IL-6 reduced; 14 days after comparison: peripheral blood of IL-6 and IL-10 concentration decreased IFN-gamma concentration increased, IL-2 and TNF-alpha concentration was no significant difference; 28 days after comparison: peripheral blood of IL-2 and IFN-gamma concentration IL-6 and IL-10 concentration decreased TNF-alpha concentration was no significant difference. The back of the conclusions the small incision organizations block plantation build rabbit VX2 renal carcinoma model high success rate, the method is simple, tumor biology and stability, easy to operate, the rabbits injury and well tolerated, it is more desirable to build rabbit VX2 method of renal cell carcinoma model. Radiofrequency ablation of renal tumors not only safe and effective for the full damage of tumor tissue, an impact on the immune system of the rabbits at the same time, can be reversed tumors causing immunosuppression, enhance the anti-tumor immunity; peripheral blood IFN-gamma, IL-2 content increased, IL -6 and IL-10 content is reduced; mechanism may be necrosis of the tumor tissue is damaged, tumor immune inhibition reduced, especially IL -6 and IL-10 immune suppression factor decreased, resulting in the body's immune system to normal activation; retention in the body due to damage of the tumor tissue in situ at the same time, the tumor damage after a large number of tumor antigen release, caused by the activation of the immune system, caused by cytokines in peripheral blood changes. Radiofrequency ablation treatment of peripheral blood IFN-gamma, IL-2 content increased, while IL -6 and IL-10 decreased, the changes of these cytokines can modulate the immune system leading to CD4 T cells, NK cells increase in the number and function enhancements anti-tumor immune enhancement.
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