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Preliminary Studies on the Correlation of β-F1-ATPase and Tumor

Author: LiCuiCui
Tutor: WangShiHua
School: Fujian Agriculture and Forestry University
Course: Biochemistry and Molecular Biology
Keywords: F1F0-ATP synthase β subunit ATP5B RNA interference
CLC: R730.2
Type: Master's thesis
Year: 2011
Downloads: 15
Quote: 0
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Abstract


The past that the F 1 F 0 -ATP synthase expressing only positioned in the mitochondrial membrane , but in recent years a growing number of studies found that its subunits are distributed in some cells the plasma membrane , called the plasma membrane ectopic F 1 F 0 -ATP synthase . Many tumor cell surface F 1 F 0 -ATP synthase , but different tumor cell surface F 1 F 0 < / sub > - ATP synthase different . F 1 F 0 - ATP synthase beta subunit functional monoclonal antibody to enhance the sensitivity of cancer cells and its drug-resistant strains of the chemotherapy drugs . This paper aims the study F 1 F 0 < / sub > - ATP synthase β subunit positioning and its expression in tumor cells the amount of difference in the effects on cell proliferation , and further explore the tumor cell membrane surface expression of the F 1 F 0 -ATP synthase functionality . For the study of F 1 F 0 - ATP synthase β subunit of the positioning in the tumor cells , we used fluorescent-labeled carrier to the β subunit with a fluorescent label . Extraction of total RNA from cells , reverse transcriptase first-strand cDNA atp5b gene was amplified by PCR and cloned into the eukaryotic expression vector pEGFP-N1 transfected cells observed by fluorescence microscopy cell surface does the presence of a fluorescent . For the study of F 1 F 0 < / sub > - ATP synthase β subunit expression differences in the effects on cell proliferation , we designed three shRNA fragment interference ATP5B protein expression , turn The cells were cultured in normal conditions and under hypoxic conditions , MTT assay was used to detect cell proliferation found shRNA1 , shRNA2 cell proliferation rate greater than 1 , shRNA3 cell proliferation rate of less than 1 .

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CLC: > Medicine, health > Oncology > General issues > Tumor pathology, etiology
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