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Background: The human glioma intracranial highest incidence of malignant tumors, infiltrative growth, surgical resection is difficult to cure, recurrence rate, long-term survival rate is low, is a difficult neurosurgical treatment, is also a hot research . Apoptosis in tumorigenesis, development, more and more attention. Clusterin and GRP78 anti-apoptotic effects were found highly expressed in a variety of tumors, but unclear gliomas, this study attempts to study their expression in gliomas and relationship with clinical and pathological features. Objective: This study aimed to explore Clusterin and GRP78 expression in gliomas and with the relationship between the clinical and pathological features: collection of North Sichuan Medical College Hospital neurosurgery surgical resection between January 2007 to May 2010 70 cases of human glioma pathology paraffin-embedded specimens for the experimental group. Including 38 males and 32 females, aged 11 to 72 years, with an average age of 45.7 years. The groups were not carried out in the case preoperative radiotherapy and chemotherapy. In another trauma underwent brain decompression 10 patients with brain tissue as a control group. Among them, 6 males and 4 females, aged 18 to 59 years old, with an average age of 35.5 years. 17 cases of 18 cases of pathological grades of glioma: Ⅰ grade 15 cases, Ⅱ grade, Ⅲ, Ⅳ 20 cases. WHO Ⅰ - Ⅱ grade divided into low-grade gliomas, WHO Ⅲ - Ⅳ class is divided into high-grade glioma group. Immunohistochemical method, using the SP method detected two groups of specimens Clusterin and GRP78 expression, double-blind method statistical content of positive cells in each specimen. SPSS13.0 software for statistical processing of corresponding data, P lt; 0.05 considered statistically significant. Clusterin and GRP78 expression in gliomas and with the relationship between gender, age, tumor size using chi-square test, Clusterin and GRP78 relationship between the use of the Spearman analysis test. Results: 1.Clusterin gliomas positive rate was 57% (40/70); positive expression in the control group was 10% (1/10), the two more significant differences (P lt; 0.05 ). Clusterin positive expression in low-grade gliomas (Ⅰ ~ Ⅱ grade) was 36% (12? 28), the positive expression in the high level of glioma (Ⅲ ~ Ⅳ grade) was 76% (28 ? 37), both statistically significant (P lt; 0.05), prompted increased with the degree of malignancy of gliomas, increased levels of tumor pathology of Clusterin positive expression rate of an upward trend. Clusterin expression and gender, age, tumor size. 2.GRP78 in human glioma positive expression rate was 51% (36/70), in the positive expression of the normal control group was 0 (0/10), both have a significant statistical significance (P lt ; 0.05). GRP78 positive expression in low-grade gliomas (Ⅰ ~ Ⅱ grade) was 30% (10? 33), the positive expression rate in the high level of glioma (Ⅲ ~ Ⅳ grade) 70% (26 ? 37), between the two was statistically significant (P lt; 0.05), prompted With higher pathological grade glioma, the higher the rate of its positive expression of GRP78. GRP78 expression and gender, age, tumor size. GRP78 expression was positive in 40 cases 3.Clusterin positive expression of the human brain glioblastoma 30 cases, Clusterin negative in 30 cases, 24 cases GRP78 expression of negative. The statistical analysis showed a positive correlation between the two. (Rs = 0.545, X2 = 20.76, P lt; 0.05) Conclusion: 1.Clusterin in human glioma abnormal expression, and the expression of the positive rate is closely related with tumor grading, with pathological grading increased expression of the more obvious, its abnormal expression may play an important role in the development of occurrence in human glioma. Clusterin expression and gender, age, tumor size. 2.GRP78 in human glioma abnormal expression is closely related to the level of its expression and tumor grading with the pathological grade increased expression of the more obvious and its abnormal expression in human glioma occur, plays an important role in the development. GRP78 expression and gender, age, tumor size. 3. Joint detection Clusterin and GRP78 can reflect tumor resistance to apoptosis degree, both synergies on the prognosis of patients, and new ideas for further study of the human brain glioblastoma.
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