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Background and Purpose tongue squamous cell carcinoma is the most common malignant tumors of the oral cavity tumors, its high incidence, prone to lymph node metastasis, and poor prognosis. Tongue squamous cell carcinoma of the traditional studies have shown that the size of the primary tumor and lymph node metastasis and recurrence is closely related to the smaller primary tumor, lymph node metastasis and recurrence of the possibility of the lower, the better the prognosis. With the widespread promotion and application of the integrated sequence of treatment with surgery, tongue cancer therapeutic levels have been greatly improved patient survival and quality of life are gradually improve, some patients and even a cure. However, we found that in clinical primary tumor smaller tongue cancer patients, the standardized surgery also experienced oral and maxillofacial surgeon case, which part of the patients was a lymph node metastasis or recurrence of the recent poor prognosis . We think this one certainly there are some factors to certain factors regulating capacity and expression differences that may exist in two different prognosis tongue cancer. Related studies show that the EGFR in head and neck squamous cell carcinoma (HNSCC) expression rate of 40% -90%, and with the poor prognosis of the disease, the progress of the disease, pathological type related. The topics to explore in the two groups of different prognosis of tongue cancer tissues EGFR, EGF expression and significance of the relationship between EGFR expression and targeted therapies, as well as the expression of EGFR, EGF and clinical factors correlation. Materials and Methods, Second Xiangya Hospital, Central South University, Hunan Provincial Tumor Hospital, Zhuzhou City Hospital 5 period from January 2005 to 2010, 32 cases of pathologically confirmed TSCC no preoperative radiotherapy, chemotherapy or other biological treatment. Collected a total of 16 cases of T1-T2, the prognosis is poor tongue cancer (A group) and 16 patients with T1-T2 of a better prognosis of tongue cancer tissue (B group). All samples of formalin-fixed, paraffin-embedded, using immunohistochemical SP method to detect factor of EGFR expression levels of EGF in the two sets of tongue cancer. SPSS17.0 software for statistical results of immunohistochemistry row rank using single-factor analysis of variance (one-way ANOVA) test with p lt; 0.05 difference was statistically significant. Results 1 of EGFR brown granular staining in the tumor cell membrane or the cytoplasm, ranging from the expression of the strength of the two sets of tongue cancer samples. 2 patients with negative expression of EGFR in 16 cases in the sample of the poor prognosis group A, four cases weakly positive, positive in eight cases, two cases of strong positive 9 patients with negative expression; 16 cases in the good prognosis group B samples, 2 cases weakly positive, five cases positive, 0 cases strongly positive EGFR expression in the group A is stronger than in group B (p = 0.012), a statistically significant difference (p lt; 0.05). 2, EGF brownish yellow staining particles mainly expressed in tongue cancer cell membrane or cytoplasm, EGF nine cases with negative expression of the 16 cases in the sample of the poor prognosis group A, 3 cases weakly positive, four cases of positive cases strong positive; 16 specimens in the good prognosis group B, 10 cases were negative, weak positive, positive in 3 cases patients with strongly positive. Compare EGF expression in the two groups of A, B p = 0.691, the difference was not statistically significant (p gt; 0.05). 3, EGFR, EGF expression in 32 cases of tongue squamous cell carcinoma gender, age, clinical stage and tumor differentiation degree of clinical factors were not statistically different (p gt; 0.05), with lymph node metastasis and without lymph node appear differentially expressed metastasis group, the difference was statistically significant (p lt; 0.05). Conclusion 1, EGFR in similar clinical stage and poor prognosis group tongue squamous cell carcinoma than in the better prognosis Group tongue squamous cell carcinoma in the expression raised. 2, EGF bad prognosis group with a better prognosis group tongue squamous cell carcinoma were expressed in similar clinical stage. The expression of EGFR and EGF in the two sets of tongue squamous cell carcinoma with gender, age, clinical stage, differentiation degree of clinical factors had no significant correlation with or without lymph node metastasis. EGFR expression differences are expected to provide a reference for the guidance of tongue squamous cell carcinoma treatment medication.
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