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Empirical Study of Treatment of Lung Cancer by Ultrosound Microbubble Uniting CD-UPRT/5-FC Suicide Gene in Nude Mice

Author: WenFei
Tutor: JiangYouFan
School: Chongqing Medical University
Course: Internal Medicine
Keywords: Microbubbles Suicide gene Lung cancer Gene therapy
CLC: R734.2
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 0
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Abstract


Objective: To investigate the ultrasound microbubble the United CD-UPRT/5-FC suicide gene in nude mice of human lung cancer cell killing effect . Methods: non-small cell lung cancer tumor-bearing mouse model , subcutaneous nodules in nude mice as long as 2 weeks after ( 0.5 ± 0.11 ) cm3 , were randomly divided into four groups . Group A was the control group , B group ( UM ) peritumoral and intratumoral injection of ultrasound microbubbles C group ( LD ) peritumoral and intratumoral injection of CD - UPRT suicide gene plasmid liposome intraperitoneal injection of 5 - FC D group ( UM D ) intratumoral and peritumoral injection of microbubbles as the carrier of CD - UPRT plasmid intraperitoneal injection of 5 - FC . Treatment were recorded every 4 days after tumor nodules change in diameter , calculate the tumor volume and tumor inhibition rate . 20 days after the mice were sacrificed remove the tumor nodules , pathological routine HE staining and immunohistochemical detection of CD protein expression . Results : The analysis of variance no significant difference between group B compared with group A tumor volume , not statistically significant ( P gt ; 0.05 ) , group C , D tumor volume was significantly less than in group A was statistically significant ( P lt ; 0.05 ) group C compared with group D tumor volume was statistically significant ( P lt ; 0.05 ) HE staining showed that in group A , group B tumor cells closely arranged , rich in blood vessels , and a large , deeply stained , mitotic C group and D group tumor cells loosely arranged , visible part of the nuclear condensation . Immunohistochemistry showed in group A , group B showed no CD protein C group and D group, the CD protein expression , intracellular staining particles in the D group significantly more than in group C , the IOD value analysis of variance statistically significant ( P lt ; 0.05). Conclusion: Ultrasound microbubble CD - UPRT cell gene transfection efficiency can be significantly increased , some in vitro human lung cancer cells in nude mice .

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