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The mole is a common gestational trophoblastic disease, abnormal proliferation of trophoblast cells, villous interstitial edema and lack of interstitial blood vessels as the main feature. According to histopathological and genetic origins, mole Partial mole and complete hydatidiform mole. View of hydatidiform \, Method 1, Real Time RT-PCR, Western blot and immune histochemical methods were used to detect the methylase Dnmts (DNA methyltransferases) in complete type mole (Complete Hydatidiform Mole CHM) and expression in normal early pregnancy villous ; 2, using methylated DNA immunoprecipitation (Methylated DNA Immunoprecipitation MeDIP) - methylation microarray analysis of complete mole and normal early pregnancy villous gene methylation status; 3, bioinformatic analysis screened differentially methylated genes and functional classification; 4, part of the differentially methylated genes detected by methylation-specific PCR (methylation specially PCR MSP). Second, the experimental results, the the Dnmt1 (p = 0.032), Dnmt3b (p = 0.009) mRNA expression level of the Real Time RT-PCR analysis of complete hydatidiform elevated Dnmt3a mRNA expression (p = 0.018) decreased and the difference was significant (p lt; 0.05). 2, Western blot analysis of the results of complete mole of Dnmt1 (p = 0.047), Dnmt3b (p = 0.006), elevated protein levels, Dnmt3a (p = 0.009) protein expression was decreased, and the normal control group in early pregnancy villi phase ratio, the difference was significant (p lt; 0.05). 3, immunohistochemical analysis of the results of complete mole the Dnmt1 (p = 0.042), Dnmt3b (p = 0.016), elevated protein levels, decreased expression of protein Dnmt3a (p = 0.027), and the control group of normal early pregnancy villi The difference was significant (p lt; 0.05). Dnmt1, Dnmt3a, Dnmt3b the complete type mole major expression in the cytoplasm of the trophoblast cells of Dnmt1 and Dnmt3b in normal early pregnancy villi expressed in the cytoplasm of some trophoblast cells in normal early pregnancy villi, Dnmt3a main expressed in trophoblast cell cytoplasm and nucleus. 4, the methylation of DNA immunization - precipitation methylation microarray analysis results in comparison with the normal early pregnancy villi, 122 gene methylation in complete hydatidiform mole, wherein the 112 gene can be mapped to the genome functional classification of genes of TRPC4, KCNT2, the of KCNQ1, KCNE1L, GABRE, KCNJ8, CLCNKA, KCNH7 ion channels; KCNT2 KCNH7, of KCNQ1, KCNJ8 with potassium transporter is closely related; UROD, GAD1 carboxylase Related; CLCNKA, KCNH7, KCNJ8 KCNQ1 voltage-gated channel. 5, methylation-specific PCR results methylation-specific PCR detection part of the differentially methylated genes, ELN in normal early pregnancy villi mainly unmethylated showed mixed A complete type mole Residues. E-cadherin, RASSF5, FGF12, SHROOM2, TIMP3 of complete mole and normal early pregnancy villi showed mixed methylation. 6 Analysis of E-cadherin, RASSF5, FGF12 SHROOM2 and TIMP3 degree of methylation (M / U), results show that E-cadherin (p = 0.043) in the complete type of complete mole and normal early pregnancy villi hydatidiform mole in the degree of methylation than normal villi (p lt; 0.05). Conclusion 1, compared with the normal early pregnancy villi of complete mole Dnmt1, Dnmt3b expression increased, decreased expression of Dnmt3a and Dnmt3a in the cytoplasm, the nucleus seldom, suggesting the occurrence of complete hydatidiform mole may be caused by overexpression of Dnmt1 and Dnmt3b, the Dnmt3a low expression of Dnmt3a caused the lost expression in the nucleus. 2, compared with normal early pregnancy villi, complete type mole 122 gene methylation and MSP verify ELN and E-cadherin methylation level higher than normal in the complete type mole early pregnancy villi, suggesting complete hydatidiform mole occurred with most of the genes related to the role of methylation in the ELN, E-cadherin hypermethylation may become completely type mole potential therapeutic targets.
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