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Effect of Downregulation of NF-κB Induced by PRMT2 on Proliferation and Apoptosis of Human Thyroid Cancer Cell Line SW579

Author: LiuMeiPing
Tutor: CaoRenXian
School: Nanhua University
Course: Endocrine
Keywords: Thyroid Cancer Cell PRMT2 cyclin D1 NF-κB Apoptosis Gene transfection Cell cycle
CLC: R736.1
Type: Master's thesis
Year: 2011
Downloads: 15
Quote: 0
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Abstract


Objectives: The Study aims to detect the expression of protein arginine methyltransferase 2 (PRMT2) in the human Thyroid Cancer SW579 cell, to construct the vector of SW579-PRMT2-V5, and to investigate the effect of PRMT2 on the growth of SW579 cells.Methods: the eukaryotic expression plasmid pcDNA3.2 /V5-PRMT2, and empty vector pcDNA3.2 /V5 were transfect into SW579 cells. The cells are selected by G418. After being transfected, proliferation of SW579 cells was observed by MTT assay. Apoptosis of SW579 cells are tested by using Hoechst 33342 staining and flow cytometry. NF-κB activation and its related genes are detected by Western blot. The expression of PRMT2 mRNA and protein are then detected by reverse transcription-polymerase chain reaction ( RT-PCR) and Western blot.Results: A low expression level was detected in human Thyroid Cancer SW579 cells. Cells with SW579-PRMT2-V5 expression were successfully established. After infection of PRMT2, the growth of Thyroid Cancer cells was inhibited significantly. Morever, PRMT2 is induced apoptosis in Thyroid Cancer cells. Also, down -regulation of cyclin D1, companied by decrease in NF–κB activation, are obseved in SW579-PRMT2-V5 cells.Conclusion: 1.Cells with SW579-PRMT2-V5 expressing were successfully established. After transfection of PRMT2, the growth of Thyroid Cancer cells is inhibited significantly.2.PRMT2 induces apoptosis in Thyroid Cancer cells. Down-regulation of cyclin D1, companied by decrease in NF–κB activation, was also obseved in SW579-PRMT2-V5 cells.

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CLC: > Medicine, health > Oncology > Internal endocrine tumors > Thyroid tumors
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