Dissertation > Excellent graduate degree dissertation topics show

The Research on Ethology and Electrophysiology of Alzheimer’s Disease by the Modulation of PPARγ Agonist

Author: GuanQiao
Tutor: WangQinWen
School: Ningbo University
Course: Biochemistry and Molecular Biology
Keywords: PPARγ Rosiglitazone Morris water maze LTP inflammatory factor drug provided with potential value for AD treatment
CLC: R749.16
Type: Master's thesis
Year: 2011
Downloads: 44
Quote: 0
Read: Download Dissertation

Abstract


Objective:To investigate the roles of the PPARγagonist rosiglitazone on Alzheimer’s disease animal model ethology and LTP (long term potentiation ),so as to offer the new way and experimental foundation to illustrate the pathogenesis of AD(Alzheimer’s disease) and further offer the theoretical basis for the novel drugs development.Methods: 1.Ethology:Male and female wistar rats (270-300g) were used in experiment. The methods of the unilateral hippocampal drug injection implemented were used for the molding and drug therapy, the MWM (morris water maze) was employed for behavioral testing .2. Electrophysiology: Fresh tansverse slices of the hippocampus were prepared from male Wistar rats (age3–4weeks). Experimental drugs was administered by superfused ACSF(Artificial cerebrospinal fluid), the extracellular electrophysiological method was applied to record fEPSP(field excitatory postsynaptic potential) and LTP and EPSP peak value was taken as index, calculateing LTP amplitude. All values were normalized to percentage relative of foundation level. Recordings were analysed using p-CLAMP (Axon Instruments, CA, USA).All values are the means±SEM. and two-tailed Student’s t test and two-way ANOVA with repeated measures were used for measurement data comparison in experiment.Results:1. PPARγagonist rosiglitazone can improve the detriment of learning and memory of rats induced by Aβoligomer and in a dose-dependent manner. 2. rosiglitazone reverse the inhibition of LTP by Aβin time and concentration-dependent manner as well,in addition,showing the difference between acute function and chronic role. 3. Rosiglitazone can overturn the inhibition of proinflammatory cytokines IL-1β、IFNγon LTP. 4. PPARγspecific antagonist GW9662 can’t block the inhibition of LTP induction by Aβ、IL-1βand IFNγ.Conclusion:Rosiglitazone can ameliorate the ability of learning and memory of AD medol rats.As a kind of high-efficiency PPARγagonist, there are two different underling mechanisms by which rosiglitazone can improve the damage of LTP induction mediated by Aβand proinflammatory cytokines IL-1β、IFNγon LTP in a PPARγ-dependent manner and PPARγ-independent manner.These have suggested that PPARγagonist rosiglitazone may be a multi-target

Related Dissertations

  1. Influence of Rosiglitazone on the Expression of PPAR γ、 NF-κB and TNF-α in Rats Model of Ulcerative Colitis,R574.62
  2. Therapeutic Effect of Rosiglitazone on Chronic Obstructive Pulmonary Disease with Diabetes Mellitus,R587.1
  3. The Effect of Simvastatin to the Appswe/PS1dE9 Transgenic Mouse with High Cholesterol Diet,R589.2
  4. PPARγ in endometrial epithelial cells and their functions preliminary study,R321
  5. The Effects of High Protein Feeding on the Brain Development of IUGR Rats,R714.5
  6. Studies on the Effects of PPARγ Induced by Wnt Signaling Pathway in NIT-1 β Cell,R363
  7. Anti-amnesic Activity of Neferine, Isoliensinine and Liensinine,R285
  8. Neurotoxicity of Amyloid β-protein and Its Possible α7 Nachr Mechanism: a in Vivo Electrophysiological and Behavioral Study in Rats,R749.16
  9. The Effects of Different Traditional Chinese Medicine Therapy on the Expression of GSK-3β in Hippocampal Region of the AD Mice Model,R285
  10. Research on Beer Foam Proteins,TS262.5
  11. Pioglitazone on focal cerebral ischemia-reperfusion injury in rats and IL-6 content of,R965
  12. XH-211 lipid-lowering , insulin-sensitizing activity was measured and its mechanism of action,R589
  13. The Mechanism Study of Buyanghuanwu Decoction on the Ability of Learning and Memory of Vascular Dementia Rats,R285.5
  14. Visual activity of the primary visual cortex of SD rats Ⅱ / Ⅲ layer inside the neuron level lateral synaptic connections plasticity,Q42
  15. NGF Regulates Expression of IDE Through PPARγ and PI3Kin PC12 and Neurons,Q42
  16. In Vivo Pharmacological Analysis of Exogenous Glycine in Mitral Cell of the Zebrafish Larvae Olfactory Bulb,R96
  17. Effect of Carotenoids and Quercetin on K562 Cell Proliferation and PPARγ Protein Expression,R733.7
  18. Effect of Thalidomide on Proliferation and Expression of PPARγ、NF-κB in Human Colorectal Cancer Cell Line HCT-8,R735.35
  19. Construction of Eukaryotic Express Vector with Mouse PPARγ Gene and Its Effect on Breast Cancer MDA-MB-231 Cells Cycle,R737.9
  20. The Neuroprotective Effect and Its Mechanism of Peroxisome Proliferator Activated Receptor-γ SCI in Rats,R651.2
  21. Cardioprotective Effects and Mechanisms of PPARγ on Injury Induced by Oxidative Stress in Developing Heart,R541.4

CLC: > Medicine, health > Neurology and psychiatry > Psychiatry > Cerebral organic mental disorder > Elderly as early as possible the old disorder
© 2012 www.DissertationTopic.Net  Mobile