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Purpose : The nervous system disease in recent years become a major problem plaguing human health , human life has brought a great deal of influence , especially with the emergence of the aging of the population , such as Parkinson's disease, epilepsy, Alzheimer's disease the incidence of diseases of the nervous system , cerebral ischemia increases seriously affect the quality of life of the elderly in old age , and therefore , many researchers dedicated to the treatment of diseases of the nervous system and mechanism . In recent years, found GFRα1 as glial cell line-derived neurotrophic factor (GDNF) receptor , GFRα1 pathogenesis of Parkinson 's disease , epilepsy , Alzheimer's disease , cerebral ischemia , and many other neurological diseases , caused a attention , has become a new focus of the treatment of nervous system diseases research . Therefore, this experiment produced a rat model of Parkinson's (PD) , observed PD striatal GFRα1 content GFRα1 gene by cloning and prokaryotic expression GFRα1 protein , for the treatment of diseases of the nervous system and pathogenesis the study provides experimental evidence . Methods: Stereotactic surgery injected 6-OHDA injection method in accordance with the two targets the right striatum of rats prepared PD animal models . PD rat model of success , by HE staining and immunohistochemical analysis , observation of the size of the neurons , morphological changes and GFRα1 expression changes , using the Image-pro6.0 analysis positive area , calculate the IOD values ??using SPSS13 .0 data one-dimensional analysis of variance . Fetal rat brain material obtained rat brain total RNA using RT-PCR amplification of the the GFRα1 full length sequence , construct the pET-30a-GFRα1 expression vector and expressed in E. coli . Results: PD rat model successfully produced , by immunohistochemistry , observed in the PD group, brain damage side GFRα1 expression is reduced , a significant difference with the control group , P lt ; 0.05 . A the GFRα1 gene of full-length sequence obtained by RT-PCR , and was expressed by E. coli , obtained by Western-blot identification of an apparent single protein western blot strips . Conclusion : GFRα1 expression in the striatum of PD rats compared with the control group decreased successfully expressed GFRα1 protein .
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