Dissertation > Excellent graduate degree dissertation topics show

Preliminary Research on Restructuring ATP7B to the Hepatocyte of Wilson Patients

Author: WuTao
Tutor: TangQiQiang
School: Anhui Medical University,
Course: Neurology
Keywords: Hepatocyte Primary culture PAS ATP7B protein Ad - ATP7B
CLC: R742.4
Type: Master's thesis
Year: 2011
Downloads: 2
Quote: 0
Read: Download Dissertation

Abstract


Background The gene mutation of human ATP7B protein is the root causes of Wilson disease (liver degeneration), the metabolism of ATP7B protein is in practice by a series of copper-protein, ATP7B gene mutations caused ATP7B protein change ,the copper ions deposit in the body ,and lead to brain and liver pathological damage. consequently as a kind of single gene hereditary disease, liver degeneration of radical method when the most ideal to liver degeneration is gene therapy.Objective To study the expression of ATP7BcDNA in the the hepatocyte of Wilson patients.Obersve the expression of ATP7B protein in various periods.Methods Hepatocyte from Wilson patients were isolated and cultured in vitro, purpose gene ATP7BcDNA was transfection the hepatocyte through viral vectors , by immunocytochemistry and enzyme linked immunosorbent assay, to detect the expression of ATP7B protein in various periods.Results 1. Hepatocyte from Wilson patients were isolated and cultured in vitro successfully,and was proved by PAS.2. The purpose gene ATP7BcDNA was proved successfully transfection into the hepatocyte.3. The synthesis of ATP7B protein was confirmed by immunocytochemistryc(ICC).4.By enzyme linked immunosorbent assay,the expression of ATP7B protein began to increase at 3h,peaked at 3-5d,then decreased gradually after 15days.Conclusin 1. Hepatocyte from Wilson patients were isolated and cultured in vitro successfully,.2. pAdxsi-GFP-ATP7B was constructed successfully.3. The purpose gene ATP7BcDNA was proved successfully transfection into the hepatocyte .4. The synthesis of ATP7B protein was confirmed successfully .

Related Dissertations

  1. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  2. Study on UCP2 Involved in the Mechanism of Regenerating Liver Against Galactosamine /lipopolysaccharide Poisoning in Mice,R575.3
  3. Study on Human Peripheral Blood Stem Cells Differentiate into Liver Cells,R575.1
  4. Morphological Characteristics of Astrocytes in EAN–a Tricellular System for Study on Neuroprotection and Neuroregeneration,R651.1
  5. Study of the Expressions of GST-π and LRP in Human Brain Glioma and the in Vitro Drug Sensitivity of Tumor Culture,R739.4
  6. Study on pH-sensitive Liposomes Loaded with Lactosyl-norcantharitin Phospholipids Complex,R943
  7. Function Test and Application of Human Fetal Hepatocyte Line L-02,R657.3
  8. The Cultivation and Cryopreservation of Mouse Multipotent Adult Progenitor Cells,R329
  9. Protective Role of Sonic Hedgehog Signaling in Primary Cultured Cortical Astrocytes Against Oxidative Stress,R741
  10. The Protective Effect of Splenectomy on the Hepatic Ischemia Reperfusion Injury,R657.3
  11. The Mechanism and Action of Glucagon-like Peptide-1 on G6Pase of Gluconeogenesis of HepG2Cells,R587.1
  12. Effect of Palmitic Acid on the Fatty Degeneration of Hepatic Cells Induced by Ehanol in Vitro,R575
  13. Protective Effects of Erythropoietin on Pancreatic Acinar Cells and Lung Tissues of Rats,R576
  14. The Promotive Effect of HGF Gene on the Proliferation of Endothelial Progenitor Cells,R329
  15. The Effect of Retinoic Acid on Rabbit RPE Cells in Vitro,R774.1
  16. Anti-angiogenic therapy inhibits liver cancer growth and metastasis, recurrence and Mechanism,R735.7
  17. Calcium-sensing receptor in rat cardiomyocytes during hypoxia and reoxygenation injury in rat and the relationship with apoptosis,R541.4
  18. Targeting STAT3 rat vascular smooth muscle cells interfere with the lentiviral vector and its effect Identification of,R543
  19. The Research of Tat Peptide-Assisting USPIO Crossing the Blood Brain Barrier Model,R-332
  20. Effects of Caveolin-1 siRNA-mediated Silencing of Caveolin-1 on Mouse FBJ-S1 Cells and Studies on GD1a-binding Molecules as Revealed by Immunoprecipitation,R73-3

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Brain diseases > Liver degeneration
© 2012 www.DissertationTopic.Net  Mobile