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Effect of Transforming Growth Factor-β on the Experession of Forkhead Box Protein-3 in the CD4~+ T Cell in Children with Kawasaki Disease

Author: LiuZuo
Tutor: LiQiu；LiChengRong
School: Chongqing Medical University
Course:
Keywords: Mucocutaneous lymph node syndrome Treg TGF-βsignaling Notch Runx
CLC: R725.4
Type: Master's thesis
Year: 2011
Downloads: 53
Quote: 0
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Abstract


Objective To investigate the effect of Transforming growth factor-β(TGF-β) and associated signaling on the expression of forkhead box protein-3(Foxp3) in children with Kawasaki Disease.Methods Twenty children with KD were enrolled into the study. The proportions of CD4~+CD25~+Foxp3high T cells (Treg) in peripheral blood were detected by flow cytometric analysis. Plasma TGF-βconcentrations were measured by enzyme-linked immunosorbent assay (ELISA). Real-time Polymerase Chain Reaction (PCR) was used to evaluate the levels of Foxp3, TGF-βand the molecules-associated TGF-βsignaling and Notch signaling. Methylation patterns of Foxp3 promoter were obtained by methylation-specific-PCR and Real time PCR.Results 1.The proportions of peripheral Treg cells and the expression of Foxp3 mRNA in KD group were significantly lower(P<0.05) 2.Decreased TGF-βconcentrations and mRNA were observed in KD group (P<0.05) 3.The expression levels of receptors and downstream target gene of TGF-βwere decreased in KD group(P<0.05). Demethylation status of the Foxp3 promoter in KD group were lower (P<0.05). 4.The expression levels of ligands of Notch and Jagged1 and Hes1 were decreased in KD group(P<0.05), while the differences being not significant only as to Jagged2 (P>0.05).Conclusions This study suggests that decreased TGF-βmight be involved in decreased Foxp3 in children with Kawasaki Disease.

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CLC: > Medicine, health > Pediatrics > Children within the science > Pediatric cardiac and vascular disease
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