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The first part of the 2007-2008 Changsha acute respiratory infections the virus spectrum objectives of the survey of hospitalized children: 2007-2008 Changsha acute lower respiratory tract infections (acute low respiratory tract infection, ALRTI) hospitalized children common respiratory virus epidemic characteristics and, oriented The prevention of area children ALRTI provide basic data and scientific basis. Objects and methods: 1, the collection at the Hunan Provincial People's Hospital in September 2007 - August diagnosis of pediatric medical center ALRTI hospitalized children nasopharyngeal aspirate (Nasopharyngeal aspirates, NPAs) specimens. 2, using RT-PCR method for detection of respiratory syncytial virus (RSV), rhinovirus (HRV), influenza virus (IFVA, B-type), parainfluenza virus (PIV1-3 type), human metapneumovirus (hMPV), coronal virus-NL63 (HCoV-NL63), coronavirus-HKU1 (HCoV-HKU1); detected by PCR adeno-virus (ADV) bocavirus (HBoV); using nested PCR method to detect polyomavirus the WU (WUPyV) and polyomavirus KI (KIPyV), and positive samples were sequenced to confirm. Epidemiological characteristics of various viruses in children ALRTI analysis. Results: 1,2007 September - 2008 August collected 1165 NPAs specimens were 871 were detected in the virus detection rate of 74.76% (871/1165), which RSV is the most common, the detection rate was 27.03 % (315/1165); followed by HRV, the detection rate of 17.33% (202/1165); PIV3 detection rate of 13.73% (160/1165); HBoV detection rate of 8.66% (101/1165); hMPV were detected rate of 6.52% (76/1165); KIPyV detection rate was 5.92% (69/1165); ADV detection rate of 4.46% (52/1165); IFVB detection rate of 3.60% (42/1165); WUPyV seized rate of 3.51% (41/1165); IFVA detection rate of 1.97% (23/1165); HCoV-HKU1 HCoV-NL63 PIV1 and PIV2 of 12 were detected, 3 parts, 2 and 7 were seized out rate of 1.03%, 0.25%, 0.17% and 0.60%, respectively. 2,763 male patients were HIV-positive were 581 cases (76.14%), the positive detection female 402 cases, 290 cases (72.13%), the virus positive rate between women and men, no statistically significant difference (χ ~~ 2 = 2.241, P = 0.134); However, the the PIV3, hMPV and HBoV positive rate of men than women, and other differences. Collect specimens of children with age from postnatal day 1 to lt; 14 years old, the average age of 15.45 ± 17.85 months. Less than 1 year old children with virus-positive detection rate was 75.37% (505/670); 1 to 3-year-old children with virus-positive detection rate was 75.57% (263/348); 72.38% detection rate of 3 to 5 years of age ( 76/105); ≥ 5-year-old detection rate of 64.28% (27/42), the positive rate between the ages statistically significant difference (χ ~~ 2 = 10.934, P = 0.027), especially in June ~ ages detected in the highest, and the respective age distribution characteristics of different viruses. 4, in the period of the study, the total virus detection rate at the Four Seasons distribution there is a statistically significant difference (χ to 2 = 12.307, P = 0.006), with the highest detection rate in winter, the the peak seasons distribution of different viruses have different. Conclusion: 1, the period of this study, ALRTI hospitalized children virus positive rate of up to 74.76%, indicating that the virus plays an important role the pathogen in Changsha area children ALRTI in. 2, this study is the first report of the 2007-2008 Changsha ALRTI hospitalized children virus spectral composition of RSV, HRV, PIV3 dominant HBoV and hMPV new discoveries in recent years have a higher proportion. , Virus detection rate statistically significant difference between the ages, particularly in June to ages detected in the highest, and the respective age distribution characteristics of different viruses; virus detection rate was no difference between men and women, have their own characteristics and different virus; virus detection rate is highest in winter, peak seasons distribution of different viruses. The years 2007-2008, the second part of the human metapneumovirus in Changsha area of ??acute lower respiratory tract infection in hospitalized children present situation analysis purposes: To understand the epidemiology of the 2007-2008 the Changsha area ALRTI hospitalized children's human metapneumovirus (hMPV) infection, clinical characteristics and genotyping, comparative PCR amplification product sequences in GenBank sequence differences, to explore the differences between the different genotypes of clinical manifestations, and lay the foundation for further prevention of hMPV. Objects and methods: 1, the collection at the Hunan Provincial People's Hospital in September 2007 - August diagnosis of pediatric medical center for ALRTI hospitalized children NPAs specimens. 2, hMPV M gene and F gene was detected by RT-PCR, and the positive amplification products connect transformed, cloned and sequenced. 3, the use of the biological software Dnastar the the hMPV reference strains measured sequences in GenBank homology comparison and nucleotide and amino acid sequence variation analysis, the use of the Mega software to complete evolutionary analysis and draw a phylogenetic tree, and genes genotyping. 4, to analyze the characteristics of the epidemiological and clinical of hMPV in ALRTI hospitalized children. Explore the differences between the different genotypes of clinical manifestations. Results: In this study, 1165 cases of NPAs specimens detected 76 cases of hMPV positive amplification products, the detection rate was 6.52%. 2, PCR products for sequencing and analysis found in GeneBank known standard strain sequence homology between 97-100%, individual base insertions and mutation, suggesting that the nucleotide of strain hMPV Changsha's little variance. hMPV Asian genotype A2 and B2 of the nucleotide sequence and the deduced amino acid sequence homology of 86.06% -87.15% 81.14% -82.46%. The nucleotide sequence and the deduced amino acid sequence homology between different Changsha strains of hMPV A2 Asian genotype were 98.91% -99.56% 98.58% -99.13%, B2 sub-genotype between 95.91% -97.98 % and 96.37% -97.38%. Evolutionary analysis showed that 76 cases of hMPV the Changsha strains can be divided into two genotypes A, B, A-type accounted for 94.73% (72/76), all of the sub-genotype A2, B type accounted for 5.26% (4/76), all of sub-genotype B2, not found in the sub-genotype A1, B1, positive children ranged in age from 20 days to 12 years old, with an average age of 15.87 months. 59 cases were males, 17 females, male to female ratio of 3.47:1, more men than women detection rate (χ 2 = 5.300 P lt; 0.05) in children under 1 year of age accounted for 63.15%, the positive rate in spring (March, April) peak in March and April, the highest, 8, 9, and 10 months, no detection. 4, in the hMPV positive specimens also detected in a variety of other virus-positive, mixed infection of up to 57.89%, wherein HBoV up, followed by RSV; single comparison hMPV and mixed with other virus-positive cases detected age, sex, fever, heat There was no difference in the drive, wheezing, vomiting, diarrhea, with or without underlying diseases, hospital days, etc. between the two. 5, cases of positive detection of clinical symptoms, signs and chest X-ray and other viral infections similar performance, including clinical diagnosis of bronchial pneumonia, bronchiolitis, acute bronchitis, bronchial asthma pulmonary infection, including bronchial pneumonia is the most common. There was no difference between the 6, compare A and B two genotypes positive cases detected age, gender, fever, vomiting, diarrhea, wheezing, rales, presence or absence of underlying disease, length of hospital stay and number of both. Conclusion: 1, hMPV is the Changsha area to hospitalized children ALRTI important viral pathogen. HMPV Changsha strain nucleotide variations 2,2007-2008 smaller, A2, B2 are two different genotypes endemic in Changsha, A2 genotype. 3, hMPV positive detection in children age mainly concentrated in less than 1 year old; men than women; peak in spring 3,4 detection rate; similar clinical manifestations and other viral infections, the major clinical diagnosis of bronchial pneumonia; with The other virus mixed 57.89% HBoV and RSV is the most common.
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