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Objective: To observe the human scar tissue exists EnMT; mice were observed for trauma healing process if there EnMT its expression in the process of wound healing. Methods: 10 each of the clinical collection of human scar tissue and normal skin specimens, specimens made of paraffin sections, F Ⅷ detected by immunofluorescence and α-SMA expression. 4 to 6 weeks of age, selected male Kunming mice 48, to establish the base of the throat skin of the back of the middle of the nearly 1.5 × 1.5cm2 full-thickness cut trauma model, respectively after injury in 3,5,7,9,12 and 14d were killed 8 mice and cut wounds and create along the tissue, cut eight mice modeling normal skin as a control group, each group of specimens taken 4 made of frozen sections, four specimens digestion were isolated and cultured vascular endothelial cells, immunohistochemical fluorescence method to detect of F Ⅷ of frozen sections and isolated and cultured endothelial cells and α-SMA expression changes. Results: Immunofluorescence test results show that the pathologic scar tissue and the presence of F Ⅷ, α-SMA-positive cells in the wound healing process. In the process of wound healing, the skin of a small amount of F Ⅷ, α-SMA double positive cells of the normal control group. Trauma group record 3,5,7,9 d and a control group statistically significant differences (P lt; 0.01), double positive cells in F Ⅷ positive cells in the proportion of single peak trend 7d when double positive cells The proportion peaked, basically returned to normal levels (p gt; 0.05) after the first 12d after record. The Conclusion: pathological scar tissue and trauma healing process EnMT showed a single peak trend in the expression of EnMT in the wound healing process. Objective: To investigate the influence of different concentrations of TGF-β1 on endothelial cells the pro EnMT role to explore EnMT pathological scar formation and wound healing mechanisms. Method: isolated and cultured human umbilical vein endothelial cells with different concentrations of TGF-β1 (10, 25, and 50 ng / ml) to stimulate the cells 72h inverted microscope after induction. Changes in cell morphology, immunofluorescence staining of F Ⅷ and α- SMA expression, RT-PCR detection of VE-cadherin (VE-Cadherin), α-SMA and type I collagen gene expression changes. Results: endothelial cells of normal control group was the cobblestone Growth, TGF-β1 stimulation of endothelial cells cobblestone morphology to spindle transformed. The immunofluorescence detection foreseeable control group containing a small amount of F Ⅷ, α-SMA double positive cells. TGF-β1 group of α-SMA, the F Ⅷ double positive cells increased significantly, and has a significant concentration-dependent manner (P lt; 0.05, P lt; 0.01). RT-PCR test results showed that TGF-β1 stimulation led to a significant reduction in the expression of endothelial cell-specific marker VE-cadherin (VE-cadherin) gene (P lt; 0.05), with the increase in TGF-β1 to stimulate concentration VE-cadherin gene expression was a significant downward trend; In contrast, the endothelial cell the Interstitial marker α-SMA and type I collagen gene expression was significantly increased (P lt; 0.05, P lt; 0.01), and has obvious The concentration-dependent manner. Conclusion: TGF-β1 stimulation of endothelial cells, cell phenotype and function showed stromal cell characteristics, suggesting that TGF-β1 to promote the role of endothelial cells to mesenchymal transition (EnMT), and its the promoting EnMT effect has obvious The concentration-dependent manner.
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