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From the Rutaceae orange Citrus reticulata Blanco cultivar peel, orange peel, peel harvested in different growing season. Qingpi divided into the Citrus reticulata flowers Qingpi two commodities specifications medicinal herbs due to harvesting and processing different way; divided into two commodities widely tangerine peel and tangerine peel herbs dried tangerine peel, herbs medicine species differences, which comes from the variant of the tea sticks of Kom C. reticulate 'Chachi' dry mature peel the Canton Chenpi in tangerine peel geoherbs. Dried tangerine peel, peel due to the same plant source ingredients, efficacy, and many other aspects of the existence of common cumulative change so that the two drugs have characteristics different harvesting period due to the secondary metabolites, such as the two drugs belong to qi drug, but tangerine peel in clinical applications longer than the spleen qi, Peel longer than the Shugan broken gas. \In this study, the research object, tangerine peel, Qingpi as traditional Chinese medicine theory as a guide around this core objective to clarify its varieties - quality - the effectiveness of \varieties of herbs indicators, representative quantitative determination of the composition and the characterization of the overall chemical mapping study and pharmacodynamics experiment, dried tangerine peel, peel quality evaluation system of building the same plant sources. Research and experimental results are as follows: 1. Comparative Study 1.1 flavonoids in this study using HPLC techniques, for the first time to establish the simultaneous determination of herbs hesperidin, naringin, rutin, naringin chemical composition of dried tangerine peel, peel, naringenin, hesperetin, nobiletin Tangeretin method. The results showed that, are peel Herbs dried tangerine peel, spent four Qingpi containing hesperidin, rutin naringin, the nobiletin Tangeretin, spent four Qingpi, the content of each component is higher than the tangerine peel (p lt; 0.01) shows that the law of the flavonoids content decreased with increasing fruit maturity; the medicine Qingpi floor fruitlet containing addition to the above four kinds of flavonoids, also contain naringin and trace amounts of naringenin, hesperetin Citrus reticulata rue naringin, hesperidin, total flavonoids than four flower peel and tangerine peel (p lt; 0.01) The nobiletin Tangeretin content of less than four flower peel and tangerine peel (p lt; 0.01) sources in variant the tea sticks Kom C. reticulata ', Chachi - Chachi - Chachi,' wide tangerine peel contains flavonoids and tangerine peel, hesperidin content is lower than the dried tangerine peel (p lt; 0.01) and other ingredients and tangerine peel the quite (p gt; 0.05) . The clustering results show that hesperidin, rutin naringin, nobiletin Tangeretin indicators compared with hesperidin or total flavonoids alone more comprehensive indicators reflect the characteristics of the herbs. 1.2 alkaloids tangerine peel and Qingpi of herbs containing synephrine representatives Alkaloids. Peel Radix synephrine content higher than tangerine peel (p lt; 0.01), shows that the trend and decreased with increasing fruit maturity; Kwong Chan Pixin, Flynn content was significantly lower than the dried tangerine peel (p lt; 0.01). Some scholars drug safety issues synephrine-containing herbs, how to ensure the safe and effective clinical drug study and formulate synephrine limited provisions pending further study, whether it should be in class the medicinal quality evaluation system. 1.3 volatile oil according to the 2010 edition of \The results show that, the main ingredient of dried tangerine peel and peel volatile oil are limonene and linalool, but from different localities, medicine varieties, different harvest time and different processing methods, as well as volatile oil instability leading to the herbs in the composition and content of volatile oil on both the big difference. 1.4 HPLC fingerprint of this study using HPLC techniques, for the first time a comparative study of orange peel, peel methanol extract fingerprint. Intuitive analysis of chromatograms, dried tangerine peel, the four flower Qingpi fingerprint difference is small, specificity or indicators peaks with the standard fingerprint similarity greater than 0.95; Qingpi on the number of peaks and peak area peel Herbs quite different, with standard fingerprint similarity in the range of 0.8 to 0.9; wide tangerine peel and tangerine peel fingerprint with standard fingerprint similarity in the more than 0.97. Cluster analysis showed that varieties from the same source dried tangerine peel, Sihua Qingpi of a class, and the different ways of harvesting and processing Qingpi a class, are derived from the tea sticks Kom C. reticulate'Chachi 'Canton Chenpi spend Qingpi a class. 2 dried tangerine peel, peel on intestinal propulsion and gastric emptying and free muscle segments in vitro comparative study of dried tangerine peel and peel as commonly qi medicine, qi stagnation and gas is mainly used for inverse embolism, the performance of abdominal distention, pain, nausea vomiting, constipation or diarrhea, gastrointestinal motility. Tangerine peel and peel herbs, the research on mice gastric emptying and intestinal propulsion comparative studies, the experimental results show that the high doses, dried tangerine peel, peel promote normal gastric emptying, and tangerine peel, peel intensity quite, but low dose does not promote the trend of normal gastric emptying; different doses, dried tangerine peel, peel could promote normal mouse intestinal propulsion, and the same dose of dried tangerine peel to promote the small intestine of normal mice to promote stronger than Qingpi. To further explore the two drugs reasonable the gas role on the differences between sites, isolated rabbit small intestine, compare tangerine peel, peel decoction, volatile oil and flavonoids part differences. The results show that, in different parts of the rabbit isolated small intestine showed inhibition for Wei Qi Yongzhi spasm pain consistent with the clinical use of drugs will be dried tangerine peel; peel decoction and total flavonoids inhibition of isolated small intestine of rabbit stronger than dried tangerine peel and volatile oil weaker than the dried tangerine peel. Differences inhibition by each major categories ingredients speculated that the difference in the efficacy of the two drugs is the result of the combined effects of the multi-component, rather than the result of a single ingredient. 3. Evaluation of the Quality of the same plant sources dried tangerine peel, peel, herbs build this study, the first comprehensive, systematic differences in the chemical composition as the starting point of a comparative study of the same plant sources dried tangerine peel, peel, herbs. The results suggest that, tangerine peel, peel-quality evaluation system construction should focus on the difference of the two drugs on the flavonoids hesperidin, rutin naringin, nobiletin Tangeretin evaluation index more comprehensive response in herbs and reflect its exclusivity; how to ensure the safe and effective clinical drug study and formulate synephrine limited provisions pending further study, and whether it should be in class medicinal quality evaluation system; volatile oil because of its closing environment, the species and its own instability, not recommendations into quality evaluation system. Meanwhile, in the quality evaluation system, recommended and peel spend peel, dried tangerine peel and Canton Chenpi single row.
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