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Curcumin in human colon cancer cell line HCT-8/VCR reversal of multidrug resistance in experimental study

Author: ZuoYong
Tutor: FuZhongXue
School: Chongqing Medical University
Course: Surgery
Keywords: Curcumin Colon Cancer MDR
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 43
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Abstract


Objective: To investigate curcumin (Curcumin) on human colon cancer cell line resistant HCT-8/VCR multidrug resistance reversal effect and possible mechanisms. Methods : MTT colorimetric determination of curcumin on colon cancer cell lines HCT-8/VCR dose-response relationship to select the appropriate dose of curcumin , curcumin cell growth curve was observed effects on cell growth and proliferation , for drug susceptibility analysis of the effect of curcumin on cell drug sensitivity ; rhodamine efflux assay of curcumin on the cell membrane drug transporter function ; RT-PCR method to detect cell multidrug resistance gene mdr1 mRNA level changes ; Western blot assay cell mdr1 gene protein product P-gp expression level changes . Results: Curcumin in 25μmol / L less on cells without significant toxicity , but significantly inhibited cell proliferation, over this concentration , the toxicity of a dose -response relationship ; and significantly enhanced cell VCR, DDP ,5-FU, HCPT chemotherapy drugs sensitivity , increase in intracellular Rh123 accumulation (P lt; 0.05) and inhibition of multidrug resistance gene mdr1 mRNA and protein expression of P-gp (P lt; 0.05). Conclusion: The results suggest that curcumin can inhibit the expression and function of resistance genes to enhance the sensitivity of cells to chemotherapeutic drugs , so as to reverse multidrug resistance of colon cancer cells , it is worth more in-depth , systematic pre-clinical studies .

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
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