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Study on Purification Of Hop Polyphenols and Its Antioxidant Activity

Author: LiuSha
Tutor: LiuYuMei
School: Xinjiang University
Course: Analytical Chemistry
Keywords: Hop polythenols Xanthohumol Antioxidant activity
CLC: TQ461
Type: Master's thesis
Year: 2011
Downloads: 150
Quote: 2
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Abstract


Hop is one of the important raw materials brewing of beer.CO2 condensate is main pro-ducts of hops.Due to the special properties of CO2,CO2 extraction contain a lots of polyphen-os.Chalcones is important a kind of polyphenos.Anti-bacterial,anti-inflammation,oxidation, inhibit the growth of tumor cells of chalcones are attittive to researchers.In this paper,hop extract residue as raw material,we focused on need for ecomnimy and study on determination of polyphenol content,extraction technology,purification technology.By statistical analysis method,paper evaluated relationship of the total polyphenol content of three main domestic hop varieties（Marco Polo,SA-1,Tsingdao flower） and alpha acid,betaacid,various mineral elements.In different areas of each variety has significant difference.According to estimate the polyphenol compounds in hop,the phenolics content were analyzed by Folin-Ciocalteu colorimetry.The ultrasonic extract-ion technology of polyphenols from hop was obtained on the basis of single fact-or and orthog-onal test.The optimal extraction conditions was determined as follows:50% ethanol as extraction solvent,temperature of extract 40℃,30 minutes of extra-ct time and 10 minutes of ultrasonic exraction time.Hop ethanol extract was material of purification.With xanthohumol extraction volumefor index,through the examination of samples-diatomite ratio,methanol concent-ration of pretreatment,solvent-material ratioof pretreatment,methanol concentration of purification,the resule indicated that opt-imal conditions were 3:1 of samples and diatomite ratio,20% of methanol concent-ration of pretreatment,1:2.5 of solvent-material ratio of pretreatment,80% of metha-nol concentration of purifycation and the purity of xanthohumol was 79.8%.The sample was recrystallize and the purity of xanthohumol was 99.2%.By using mel-ting point apparatus,ultraviolet-visible spectrometry（UV）,high perfor-mance liquid chromatography（HPLC）and nuclear magnetic resonance spectrometry（1HNMR）,the sample was identified as xanthohumol.We compared new purification conditions wit- h the previous that equipment requirements steps was simple and cheap.Purification results were satisfactory and accorded with industrial requirements.The antioxidant activities of hop ethanol extract using were evaluated by vitro DPPH radicals,hydroxyl radicals,super-oxideanion radicals,ferric thiocyanated method （FTC） and thiobarbituric acid method （TBA）.All hop samples showed varying degrees of antioxidant capacity.Among experimentation of DPPH·and OH·,polyphenol contents （0.04-0.25mg/mL）presented positive correlation.Clearing of super-oxideanion radicals,FTC and TBA polyphenol has never expressed showed relation.Totaling 101 samples from different species in different regional were determine on total polyphenol,total flavonoids,alpha acid,beta acid and mineral elements.The study showed that hop polyphenols and hop acidity of bitterness content became direct ration.Flavonoids of Tsingdao flower was the most proportion to compare with SA-1,Marcopolo.Part of the mineral element of the hop of different varieties,from different habitats are significantly different.In addition the total polyphenol content andmineral element content there was no significant relationship.

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