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Red-seed Watermelon seeds are rich in protein using alkali fusion protein isolate extracted from the red seed watermelon seeds in the four factors that affect the protein extraction rate by orthogonal experiment ( solid-liquid ratio , temperature , pH, extraction time ) optimize the solid-liquid ratio of 1:25 , temperature 40 ° C , pH 9.5 , extraction time 1.5h protein extraction rate of 94.35% . The results show that the Red-seed Watermelon seed kernel protein processing characteristics , temperature, pH, sodium chloride concentration has a significant impact on the processing characteristics of the protein . When the temperature was 40 ° C , saturated protein solubility highest 18.39mg/mL, emulsifying capacity and emulsion stability of the protein are best when the temperature is 55 ° C , of 17.9m ~ 2 / g and 97.19% , respectively , when temperature of 50 ° C , the water holding capacity of up to 2.33g / g, when heated 45min , the best oil absorption of 6 mL / g and a temperature of 80 ℃ , the oil absorption maximum of over 6.25 mL / g ; when the pH is 4.5 . the saturated protein solubility minimum 1.06mg/mL , when the pH is 9 , preferably , the emulsifying capacity and emulsion stability , respectively, for 17.9m ~ 2 / g and 98.04% , when a pH of 7 , the water holding capacity of up to 3.58g / g; when the sodium chloride concentration of 0.4mol / L , the saturation solubility of the protein up to 17.32mg / mL, when the sodium chloride concentration of 0.6mol / L , emulsifying capacity and emulsion stability have reached the maximum , respectively, 11.27m ~ 2 / g and 70.53% , the water holding capacity has reached the highest value of 2.34 g / g . Seed watermelon seed kernel protein hydrolysis red alkaline protease optimize the enzymatic hydrolysis process : a temperature of 55 ° C , pH 10 , the substrate concentration of 4% , the amount of enzyme to 20400U / g , hydrolysis time of 2h . The optimal enzymatic process , the clearance of protein degree of hydrolysis (DH) and the product of the hydroxyl radical (· OH) 35.09% and 94.28% , respectively . Enzymatic liquid chromatography SepHadex G-25 to get the two components I and II . Component II clearance rate than · OH component I , collected fraction II antioxidant activity evaluation component II of superoxide radical (· O2-), hydroxyl radical (· OH) and mice the liver homogenate since malondialdehyde (MDA) has a clear inhibitory effect .
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