Dissertation > Excellent graduate degree dissertation topics show

Quality Evaluation of Dendrobium Tissue Culture

Author: XinTian
Tutor: DiYanJun
School: Liaoning University of Traditional Chinese Medicine
Course: Pharmacognosy
Keywords: The iron Dendrobium ( candicum , ) Dendrobium candidum embryoids Dendrobium officinale tissue culture Pharmacognosy Pharmacodynamic
CLC: S567.239
Type: Master's thesis
Year: 2011
Downloads: 368
Quote: 0
Read: Download Dissertation

Abstract


Objective: Dendrobium candidum Dendrobium mainstream commodity herbs versatile, high demand, special growth conditions, resources are scarce, expensive, China Plant Red Data Book \In order to solve the problem of shortage of resources of Dendrobium officinale, tissue culture of Dendrobium candidum and Dendrobium candidum quality evaluation research aims to provide a theoretical basis for the to tissue culture Dendrobium candidum alternative Dendrobium candidum meet market demand. Paper tissue culture of Dendrobium candidum pharmacognosy research, analysis and comparison of the morphology and chemical composition with the commercially available Dendrobium officinale (trade Dendrobium) to explore of Dendrobium candidum commercially tissue culture Dendrobium the quality correlation. Paper tissue culture of Dendrobium candidum system pharmacodynamic study Dendrobium candidum with commercially available tissue culture Dendrobium anti-fatigue, anti-anoxia, anti-radiation, in vitro antioxidant and anti-tumor effects in comparison to correlation to determine the pharmacodynamic characteristics of both, to provide scientific information for the development of varieties. Materials and methods: 1. Material: Dendrobium candidum embryoid of Dendrobium candidum tissue culture and commercially available iron maple bucket. 2 methods: (1) through digital photographs and microscopic photo phase Dendrobium candidum embryoid Dendrobium plantlets and commercially available tin candicum morphological comparison, including comparison of its Characters and microscopic characteristics ; (2) by UV spectrophotometry, ninhydrin post-column derivatization and atomic absorption spectrophotometry chemical composition analysis of three kinds Dendrobium officinale herbs, including polysaccharides, alkaloids, cellulose, amino acid content of trace element. (3) by in vivo experimentation (including ultraviolet spectrophotometry, diacetyl - oxime method, etc.) on the tissue culture of Dendrobium candidum anti-fatigue, anti-anoxia, anti-radiation and by in vitro method (including the removal of the hydroxyl radical law pyrogallol autoxidation method, MTT assay) tissue culture of Dendrobium candidum antioxidant and anti-tumor effect. The results: 1. Morphological study: (1) macroscopic characteristics: three kinds Dendrobium officinale herbs due to different growth conditions, macroscopic characteristics are quite different, but the smell is consistent. (2) microscopic characteristics: Dendrobium medicinal powder characteristics, smaller calcium oxalate crystal beam, Dendrobium embryo, stem epidermal cells, non-glandular hairs, calcium oxalate crystal beam Dendrobium plantlets powder characteristics No significant microscopic characteristics like body powder. Chemical composition comparative study: (1) The polysaccharide content: the content of Dendrobium highest (16.67%) gt; Dendrobium candidum embryoids (11.57%) gt; tissue culture of Dendrobium officinale (6.42%). (2) the total alkaloid content: the the Dendrobium highest (.08570%) gt; Dendrobium candidum embryoids (0.01662%) gt; tissue culture of Dendrobium officinale (0.00734%). (3) cellulose content: the the Dendrobium highest (61.39%) gt; of Dendrobium candidum plantlets (49.22%) gt; Dendrobium candidum embryoids (43.73%). (4) The content of trace elements: of Dendrobium candidum embryoid Dendrobium candidum plantlets contained trace elements except Ca, Mg, Sr slightly lower than commercially available iron candicum other trace elements content above the Dendrobium The highest content of tissue culture of Dendrobium candidum. (5) The amino acid content: of Dendrobium candidum plantlets (14.61%) and the content of the Dendrobium candidum embryoids (8.62%) is much higher than Dendrobium (2.11%). Pharmacodynamic comparison study: (1) anti-fatigue: tissue culture of Dendrobium candidum can significantly prolong the mice burden swimming survival time to ease the problem of reduced blood sugar levels in rats after exercise to alleviate post-exercise lactic acid content of whole blood and blood urea increase of the nitrogen content, a decrease of liver glycogen, muscle glycogen consumption to achieve the effect of anti-fatigue. (2) hypoxia tolerance: tissue culture of Dendrobium candidum can significantly prolong the survival time of mice in normobaric hypoxia conditions, the the nitrite poisoning survival time of acute cerebral ischemia survival time and dose-dependent relationship, suggesting that a certain degree of anti-hypoxia effect. (3) anti-radiation: tissue culture of Dendrobium candidum can increase the number of white blood cells in the peripheral blood of mice after radiation and restore the spleen index, thymus index, prophylactic administration of SOD activity in mice than the model group, MDA content is low in the model group, the tissue culture of Dendrobium candidum can significantly reduce the radiation on mice damage. (4) in vitro antioxidant: tissue culture of Dendrobium candidum decoction of hydroxyl radicals and superoxide anion radical scavenging effect on lipid peroxidation inhibition produced to prove that its antioxidant capacity. (5) The in vitro anti-tumor cultures decoction fine polysaccharides have a certain inhibitory effect on tumor cells (A549, SW480, Hela, QGY): Dendrobium candidum organization, but not related to the concentration of the water fried concentration of 20 mg · mL-1 and refined polysaccharide concentration 500μg · mL-1 as the the best inhibitory concentration confirmed for the adjuvant treatment of lung cancer, colon cancer, cervical cancer and liver cancer in clinical. Conclusions: 1 In this paper, two kinds of tissue culture of Dendrobium candidum patented products provide by Shanghai Tang Zhensheng biotechnology Limited is induced on the the adult stem segments of the original old plants dormant buds, implanted by the shoot tip explant inoculation to liquid obtained on the medium and solid medium (its specific culture conditions confidentiality); the selected reference drug Dendrobium dried wild Dendrobium candidum made without doping any other ingredients can be used as a wild Dendrobium representatives. This article is the first time this patented product commercially available Dendrobium quality evaluation studies. In this paper, for the first time on tissue culture of Dendrobium candidum with commercially available iron maple bucket morphological comparison. And cultured and wild species due to different growth conditions and growth time on the macroscopic characteristics quite different, but consistent source smell the same; microscopic features of tissue culture of Dendrobium candidum short incubation time, microscopic characteristics than less. Firstly systematically on tissue culture of Dendrobium candidum with commercially available iron maple bucket chemical composition comparison. The results showed that the two kinds of tissue culture of Dendrobium candidum herbs short incubation time, slightly lower than the commercially available Dendrobium medium rich in nutrients, amino acids and trace elements contained in the polysaccharide, alkaloid content and other nutrients far above the Dendrobium, the degree of fibrosis, confirmed the tissue culture products contain high nutrients, to meet the health needs of the human body, we can see that the chemical quality of the herbs in the two kinds of tissue culture of Dendrobium candidum not lower than the wild species. Firstly tissue culture of Dendrobium candidum several pharmacodynamic study. In the the antioxidant and antitumor trial, for the first time using the tissue culture of Dendrobium candidum decoction experiment. Pharmacodynamic tissue culture experiments confirmed Dendrobium candidum anti-fatigue, anti-anoxia, anti-radiation, anti-oxidation and anti-tumor have a certain effect, can be used in the clinical treatment or adjuvant therapy, tissue culture Dendrobium further pharmacological The activity study provides a theoretical basis. Dendrobium candidum. Tissue culture production cycle is short, simple mode of production, production conditions controllable, uniform product quality, large output, lower prices, etc., can be used in the pharmaceutical industry not only in modern life, but also in health food , beauty skin care products play a role, it also provides an effective way to solve the problem of scarce resources of wild Dendrobium candidum.

Related Dissertations

  1. Of Guben Zhikepingchuan particles pharmacodynamic study,R285.5
  2. Studies on Preparation Technology, Quality Standard and Preliminary Pharmacodynamic of Qixian Granules,R285
  3. Studies on Extracts from Huidouba and Its Preparations,R284.1
  4. Cordyceps Gracilis Fermentation Process and Pharmacodynamics,TQ920.1
  5. The Toxicology and Pharmacodynamic Study of Crude and Processed Homalomena Gigantea Engl,TQ461
  6. Study on Pharmacodynamics and Pharmacokinetics of Praziquantel Injection in Mice,S859.1
  7. Selection and Study on Toxicology, Pharmacodynamics of Yandureqing Liquid Injection for Chinese Veterinary Medicine,S853.7
  8. Study on Processing Technology and Efficacy Evalution of 30% Cangersu Emulsifiable Concentrate,S482.2
  9. A Comparison of the Efficacy of Continuous Administration Using Minipump and Fractional Administration of Antibiotic in Treating Lower Respiratory Tract Bacterial Infections,R96
  10. Study on Insulin Phytosome and Self-microemulsion,R94
  11. Ibuprofen lysine capsules Preparation and Quality Standards,R944
  12. Epinephrine on epidural levobupivacaine pharmacodynamic and pharmacokinetic effects,R614
  13. Study on Chronopharmacokinetics & Chronopharm Acodynamics of Injuryed Liver in Rats after Administration of Xiaoyo Powder,R285.5
  14. Preparation and Evaluation of Chansu Cataplasmata,R283
  15. Preliminary Study on Pharmacodynamics of the Folkremedy of Digugao,R285
  16. Preparation and Evaluation of Shen’e Capsules,R286.0
  17. Huosu Qingre Particles Beans Pharmacody-namics Research and Studies on the Long-term Toxic-ity in Young Rats,R285.5
  18. Preparation and Pharmacodynamic Study of Compound Glycyrrhiza Flavonoids Cream,R285
  19. Study on Anti-angiogenesis Effects of Albizia Different Extraction Fractions,R285
  20. Studies on the Effects and Mechanisms of QingXiangRuKang Particle on Hyperplasia of Mammary Gland,R285.6
  21. Experimental Study of Scutellarin in the Treatment of Hepatic Fibrosis,R285.5

CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Herbal > Perennial > Other
© 2012 www.DissertationTopic.Net  Mobile