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Objective: To observe the different doses of levodopa (Levopoda, LD) for monocular deprivation amblyopia rat visual cortex nerve cell morphology, and to explore the possible mechanism of its treatment of amblyopia. Methods: 36 14-day-old SD rats (Central South University animal department) were randomly divided into three groups, namely amblyopia control group, low-dose group and high dose groups of 12. Right eyelid suture after 4 weeks, cut eyelid suture line flash visual evoked potential (F-VEP) examination to determine the form deprivation amblyopia model is successful, low-dose group and high dose group were at a concentration of 20 mg / kg, 80 mg / kg was administered levodopa, amblyopia saline control group, once daily for 4 weeks after the rats were sacrificed cerebral visual cortex OK hematoxylin - eosin (HE staining), light were observed by HE staining of nerve cells in the visual cortex V1 morphology, TUNEL assay for quantification of each rat visual cortex neuronal apoptosis. Results: 1. F-VEP latency of the P2 wave of change: before administration the rats deprived eye F-VEP of the P2 wave latency was significantly longer than those without the deprived eye (P lt; 0.05), the difference was statistically significant, N2P2 wave, P2N3 amplitude difference was not statistically significant (P gt; 0.05), is not deprived eye, deprived eye P2 wave latency and N2P2 wave, P2N3 amplitude difference between groups was not statistically significant (P gt; 0.05) 2 light microscope HE staining of nerve cells in the visual cortex V1 morphological changes: the rats are not deprived eye side Pizhi corresponding number of nerve cells in V1 and morphology were normal; amblyopia group deprived eye compared with non-deprived eye, nerve significantly reduced the number of cells, smaller size, reduced cytoplasm, cytoplasmic processes shorter or disappear, the nucleus becomes smaller, darker, and visible dissolution, condensation, fragmentation and other phenomena, showing class apoptosis; small dose group deprived eye compared with non-deprived eye nerve cell number and morphology only slightly changed. High-dose group deprived eye compared with non-deprived eye no significant change .3. TUNEL assay of nerve cells in the visual cortex V1 apoptosis and counting: the rats are not deprived eye V1 region corresponding side Pizhi found only a small amount of staining green fluorescence-positive neurons deprived amblyopic eye than the control group did not deprive the eye area V1 corresponding positive side Pizhi significantly increased the number of nerve cells, the difference was statistically significant (P lt; 0.05), low dose group deprived eye is weak, as the control group deprived eye area V1 corresponding positive side Pizhi reduce the number of nerve cells, the difference was statistically significant (P lt; 0.05), high-dose group deprived eye smaller dose group deprived eye area V1 corresponding positive side Pizhi reduce the number of nerve cells, the difference There was statistically significant (P lt; 0.05). Conclusion: a rat form deprivation amblyopia F-VEP latency of the P2 wave, amblyopia side Pizhi corresponding decrease in the number of nerve cells in V1, smaller size, was the class of apoptosis. (2) exogenous levodopa can improve rat form deprivation amblyopia side Pizhi V1 region corresponding classes of nerve cells apoptosis. 3 amblyopia reduce levodopa-induced central nervous system cell morphology and visual changes in the number may be through inhibition of the visual cortex neuronal apoptosis in class, and thus participate in the development of the visual system and the remodeling process.
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