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Right Nr2f2 and Nov role in osteoblast preliminary study

Author: WangZuo
Tutor: ChenMeiHong
School: Beijing Union Medical College
Course: Biochemistry and Molecular Biology
Keywords: Nr2f2 Nov MC3T3-E1 cell MG-63 cell cell proliferation
CLC: R346
Type: Master's thesis
Year: 2011
Downloads: 20
Quote: 0
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Abstract

RNA interference (RNAi) is a sequence-specific posttranscriptional gene silencing process mediated by double-stranded RNA. With the help of particular nuclease, dsRNA was processed into small interfering RNAs (siRNAs) which subsequently cleave and destroy the cognate mRNA. This widespread eukaryotic surveillance mechanism which mediate anti-virus invasion, transposon silencing as well as gene expression control has successfully been used in the study of gene function and signal transduction. As the study achieved, RNAi has been applied to functional genome research and using siRNA library to high-throughput screen more and more catch researcher’s eyes.We use the random siRNA library to screen for siRNAs that can enhance cell proliferation and obtain siRNA-A281 which promote MC3T3-E1 cell proliferation. Mouse genome expression profile shows that cells proliferation were accelerated when transfect siRNA-A281. Meanwhile, the expression of Nr2f2 was boosted; indicating Nr2f2 possible has a role in MC3T3-E1 cell proliferation.Nr2f2 (nuclear receptor subfamily 2, group F, member 2), also known as COUP-TFⅡ(Chicken ovalbumin upstream promoter transcription factors 2) are orphan members of the steroid/thyroid hormone receptor superfamily. Nr2f2 protein is ligand induced transcription factors that mediate various genes expression. COUP-TFⅡplays a critical role in controlling the development of a number of tissues and organs including heart, blood vessels, muscles and limbs. However, no evidence has been reported to show Nr2f2 can enhance cell proliferation.To confirm whether Nr2f2 can accelerate cell proliferation, we perform a series experiments and found that Nr2f2 accelerates mouse pre-osteoblast proliferation mainly through increasing the proportion of cells in S phase.We also elucidate the cell proliferation-enhancing function of Nov which encodes a secreted signal protein. The current understanding of Nov shows that it has a anti-proliferative activity. However, our results show that Nov can accelerate MC3T3-E1 cell proliferation when over-expressed. Meanwhile, we found a point mutation of mRNA in human osteosarcoma cell line MG63. We hope these results can provide some clues to the gene mechanism study. To sum up, we use random siRNA library to screen for siRNAs that can enhance cell proliferation and found Nr2f2 and Nov has a role in MC3T3-E1 cell proliferation.

Right Nr2f2 and Nov role in osteoblast preliminary study

Abstract

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