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objective To discuss the expression of HO-1, TNF- in bleomycin-induced ulmonary in the expression and the effect of matrine on them.Methods 90 healthy male SD rats were randomly divided into five groups, the normol control group, model group, Prednisone group, Matrine low dose groupMatrine middile dose group.Pulmonary fibrosis model is made by the way which is given blecomin(5 mg/kg) through trachea.The normol control group is given saline by the same way. From 1st day after modeling, the rats were fed prednisone acetate (0.56mg/kg/d) , different dose matrine (50mg/kg/d,100mg/kg/d) and saline. 7,14,28 days at the time of modeling,each group 6 rats were randomly killed and get the lung tissue, and detectd lung tissue malondialdehyde (MDA) level, hydroxyproline (HYP) content, in lung tissue, HO-1 and TNF- mRNA is measured by RT-PCR, and protein content of HO-1 is measured by Western blot, ELISA detect the expression of TNF- .The lung tissue biopsy sections are detected by HE and Masson staining pathology.Results Biopsy sections revealed: In model group, inflammatory response is the main performance at 7d, and a lot of lung fibroblast can be found in addition to inflammatory cells, the collagen can be found by the masson staining,that mean pulmonary fibrosis to begain at 14d. at 28d, more lung fibroblast can be found and alveolar is squeezed, the collagen can be found more clearly, Pulmonary fibrosismodel that has been formed. Pathological changes in the treatment group and model group is similar, but less than the model group, of which matrine middle dose treatment group and prednisone treatment group reduce significantly.MDA and HYP content: 7d,14d,28d MDA and HYP content in model group, prednisone group, matrine low dose group and matrine middile dose group was higher compared with normol control group (p<0.05), the model group was most obvious (p0.01), after matrine and prednisone inteevented, both of content become lower ,the change was most obvious in 28d;compared with model group, matrine middle dose group and the prednisone group most significantly change(p 0.05).RT-PCR Western blot and ELISA showed: In the normal lung tissue, TNF-and HO-1 is expressed, HO-1 expression is low. Pulmonary fibrosis, lung tissue levels of both significantly increased compared with normal tissue (p<0.01). After drug intervention, compared with model group, The levels of both were decreased in matrine low dose groups, matrine middle dose group and prednisone group (p<0.05),but the matrine middle dose group and the prednisone group was more significant (p<0.01).Conclussions 1 Matrine has the role of anti-fibrosis and the role has in a dose dependent manner.2 The mechanism of matrine in resistance to pulmonary fibrosis may regulate oxidative antioxidant imbalance, lower lung tissue MDA, HYP content, inhibition of TNF- production and the change in the expression of HO-1 related to the level
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