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Bio-informatic Analysis of Evolution and Transcription of Karyopherin β Superfamily

Author: WangZuo
Tutor: TaoTao;JiZhiLiang
School: Xiamen University
Course: Cell Biology
Keywords: Nucleocytoplasmic transporter receptor Phylogenetic Expression regulation
CLC: Q2
Type: Master's thesis
Year: 2008
Downloads: 56
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Abstract


The nucleocytoplasmic transporter receptor family members (karyopherinβ.superfamily) mediates most biological macromolecules in eukaryotes across karyotheca transport . At present it has been found that the cells under different physiological developmental conditions nucleocytoplasmic transport a variety of substrates regulatory mechanism , but for the family members of the system evolution and the regulation of gene expression is still poorly understood , so we bioinformatics methods for these unknown areas of preliminary exploration . Phylogenetic trees were constructed , we found 10 yeast nucleoplasm nucleoplasm eight species ( yeast , C. elegans , Drosophila , zebrafish , Xenopus , jungle fowl , mice and humans) transporter receptor family members transporter the receptor the ancestors (KAP95, KAP123, KAP114, KAP120, KAP122, CRM1, MSN5, LOS1, MTR10 and CSE1) and Drosophila XP06 ancestor 11 ancestors directly evolve into higher eukaryotes body corresponding orthologs ; while another four ancestors (KAP104, NMD5/SXM1 PSE1) yeast and nematodes XPO7 ancestors in the evolutionary process happened gene duplication or deletion of intron event , eventually evolved into the appropriate collateral congeners . Meanwhile, we studied the expression and distribution of nucleocytoplasmic transport receptor members , found that the vast majority of family members are ubiquitously expressed in various tissues and organs of the human body , but the expression of differences : the nucleocytoplasmic transporter receptor in mouse embryonic development expression levels are precisely regulated . The key to further study the mechanisms of gene expression and regulation of the family , found that most of the family members of the promoter region presents a housekeeping gene regulation , Sp- 1 , C / EBP , MAZ and NF-1 may be the start or regulation of gene transcription factor. These findings will help us further understand the trend With the increasing complexity of eukaryotic physiological functions , the the the nucleocytoplasmic transporter receptor family members in the function and expression regulation more diverse with precision .

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