Dissertation > Excellent graduate degree dissertation topics show

Spectroscopic and Photoelectrochemical Properties of Biomacromolecules-Semiconductor Nanoparticles Composites

Author: ChenQiaoLin
Tutor: LinZhongHua；ZhouJianZhang
School: Xiamen University
Course: Physical and chemical
Keywords: semiconductor nanoparticles (quantum dots) DNA proteins
CLC: TB383.1
Type: Master's thesis
Year: 2008
Downloads: 94
Quote: 1
Read: Download Dissertation

Abstract


In recent years,the integration of semiconductor nanoparticles and biological systems has attracted a great many concerns in biology and medicine field. Investigations of biomacromolecules-semiconductor nanoparticles conjugates（composites） will provide significant theoretical foundation for the biological study and medical diagnosis and assist the construction and operation of nano-molecular devices and biosesors.Besides,semiconductor nanoparticles as fluorescence biological probes play an important role in biological labeling, bio-imaging,and biological detection.Accordingly,the emphasis of this thesis is focused on investigating the biomacromolecules-semiconductor nanoparticles conjugates and semiconductor nanoparticles as fluorescence biological probes.These works can be divides into two parts:1.DNA/semiconductor nanoparticles composites were constructed utilizing DNA as the stabilizers and templates.The influence of DNA on the spectroscopic and phtoelectrochemical properties of the composites was investigated,and the interactions between DNA and semiconductor nanoparticles were discussed.2.Different kinds of fluorescence biological probes based on semiconductor nanoparticles were fabricated,and their photo-induced electron transfer with redox proteins were studied by spectroscopic characterizations.The influencing factors on the interactions were further investigated.The main results are summarized as follows:1.The interactions between DNA and semiconductor nanoparticles（1） In DNA/CdS nanoparticles composites,CdS nanopaticles were intergrated with DNA through electrostatic affinity;DNA scaffolds did not affect the energy band of CdS nanoparticles;CdS nanoparticles fabricated by ssDNA and dsDNA templates possessed a higher density of surface states than that by Na4P2O7,which enhanced the fluorescence while restrained the photoelectrochemical response of CdS nanoparticles; the density of surface states in the composites fabricated by ssDNA was higher than that by dsDNA.（2） In DNA/ZnS nanoparticles composites,DNA scaffolds quenched the fluorescence of ZnS nanoparticles.Besides,the quenching due to ssDNA was stronger than that due to dsDNA.2.Photo-induced electron transfer between redox proteins and semiconductor nanoparticles（1） There were electrostatic interactions between cytochrome c and semiconductor nanoparticles.Ferricytochrome c captured the excited electrons of CdS and ZnS nanoparticles under photo-irradiation and was reduced to be ferrocytochrome c displayed by UV-Vis spectra,which diminished the radiative recombination of the holes and the excited electrons and quench the fluorescence.The fluorescence quenching efficiency of cytochrome c varied in different semiconductor nanoparticles systems.P2O74- and L-cysteine located at the nanoparticles/cytochrome c interface may act as "bridges" for the electron transfer,while in the systems protected by DNA, there was little electron transfer between cytochrome c and nanoparticles.（2） The interactions between horseradish peroxidase（HRP） and semiconductor nanoparticles included two parts:one is the hydrophobic interaction,which can enhance the fluorescence of semiconductor nanoparticles;the other is the electron transfer between Fe-protoporphyrin and semiconductor nanoparticles,which can quench the fluorescence of semiconductor nanoparticles.But the effects of electron transfer may be very small.

Related Dissertations

  1. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  2. Treatment Efficiency of Entecavir and TACE in Patients with Primary Heptatic Carcinoma of the HBV Copying,R735.7
  3. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  4. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  5. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  6. Screening of Proteins Interacting with Streptococcus Equi Ssp. Zooepidemicus M-Like Protein in Swine Vascular Endothelial Cells,S852.611
  7. Study on Processing Functionalities and Application of Bovine Plasma Proteins,TS251.1
  8. Primary Studies on Transcriptome and Population Genetics of An Invasive Weed Mikania Micrantha,S451
  9. Investigation of Cereal Cyst Nematode on Wheat in Jiangsu Province and the Sequence Analysis of rDNA-Its Region from Jiangsu Populations,S435.121
  10. Gene Prediction and Sequence Analysis of Insect Odorant Binding Protein, Chemosensory Protein and Ryanoding Receptor in Eight Transcriptome Data,S433
  11. Bioinformatics Analysis of Snare Proteins and Functional Characterization of Mgsec22 in Magnaporthe Grisea,S435.111.41
  12. Studies on Extracting Mitochondrial DNA and Gene Expressions in Brassica Napus L,S565.4
  13. Studies on the Mechanism and Recovery Measures of Hyperhydricity of Pyrus Calleryana Decne in Vitro,S661.2
  14. In Vitro Rapid Propagation System of Peach Yu Dwarfing Rootstock No.1 and Optimization of Its SSR-PCR System,S662.1
  15. Effects of 5-Aza-2-deoxycytidine on DNA Methylation and Expression of p16 and MGMT Gene in Lung Cancer Cell Line SPC-A-1,R734.2
  16. Methylation of p16 Gene in Plasma and Tissues from Non-small Cell Lung Cancer Patients, Demethylation and the Biological Behavior of Lung Cancer Cell and Transcription of p16 Gene,R734.2
  17. DNA Prime/Protein Boost of Japanse Edcephalitis Virus NS1 Protein to Improve Immune Response in Mice,R392
  18. Epidemiological Investigation of TS and IHHN and Preliminary Study on Interaction of Structural Proteins of TSV VP1 and VP3,S945
  19. Molecular Phylogeny of Rhus Gall Aphids (Hemiptera: Aphididae: Pemphiginae) Based on DNA Sequences,S899.4
  20. The Preliminary Study of the pcDNA3.1(+)-SjP14 Nanometer Microballoon-DNA Vaccines,R392.1
  21. Effect of SO2 Exposure on DNA Methylation Polymorphism in Arabiadopsis Thaliana,Q943

CLC: > Industrial Technology > General industrial technology > Materials science and engineering > Special structural materials
© 2012 www.DissertationTopic.Net  Mobile