Dissertation > Excellent graduate degree dissertation topics show

Study on the Types of Immunocytes Infiltrated in Intimal Arteritis in Rejected Kidney Allograft

Author: SunHuaJun
Tutor: WuShan
School: Jilin University
Course: Pathology and Pathophysiology
Keywords: Kidney transplantation Antibody-mediated rejection Intimal arteritis T-lymphocyte Macrophage
CLC: R699.2
Type: Master's thesis
Year: 2009
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


Background. Acute allograft kidney rejection was consisted of T-cell mediated rejection and antibody mediated rejection. People know T-cell mediated rejection much better than antibody mediated rejection because that antibody mediated rejection accounts for only 0~8% of the total recipients population after kidney transplantation. Antibody mediated rejection showed diversify histological features contained Intimal arteritis, glomerulitis and peritubular capillaritis. But the immunophenotype of the cells which infiltrated in the Intimal arteritis and glomerulitis was still not been clarified. Then we take use of four excised kidney allograft which suffered acute rejection. to identify the cellular infiltrates in intimal arteritis and glomerulitis.Methods. We obtained four allografts removed from renal transplant recipients who had been diagnosed by clinical criteria and/or biopsy as having acute allograft rejection before their transplants were resected. Then the specimens were performed HE staining and detected C4d deposition by immunohistochemistry, for the aim of diagnosis. Based on the diagnosis of antibody mediated rejection we clarified the immunophenotype of the cells in the intimal arteritis and glomerulitis by immunohistochemistry in serial sections of the resected kidney. Moreover, in aim to clarify the relationship of position between the immunocytes and endothelial cells, double staining of immunohistochemistry was performed with the antibodies of lysozyme(macrophages) and CD34(endothelial cells).Results. These four kidney allografts showed classical pathological features of antibody-mediated rejection,we found infarction at different extension, and we can see intimal arteritis, glomerulitis, and peritubular capillaritis beside the infarction area. Immunohistochemistry for C4d showed that all of these four cases were positive fpr C4d staining according to the latest diagnose standard, finally all these cases were diagnosed as suffered acute antibody mediated rejection. The result of immunohistochemistry for the immunocytes showed that in the intimal arteritis, the most important cellular infiltration came from macrophages and T-lymphocytes which took percentage of 39.8±10.5% and 32.3±14.4% respectively. There was no significant difference between the two kinds of cells, but their percentages were higher than those of B cells, plasma cells and neutrophils. Even the T cells took a huge percentage for the cellular infiltration in the intimal arteritis, the CD8 positive cells did not account most of them, the CD8 positive rate was only 14.9±8.3% and was much lower than that of CD3 positive cells. At the same time, the NK cell can appear in the intimal arteritis too. At last, result of double staining of immunochemistry showed that the infiltrated cells were indeed under the endothelial cells, not in the lumens. The cells which infiltrated in the glomerulitis were macrophages and neutrophils.Conclusion. All these four cases were diagnosed as suffered acute antibody mediated rejection. Both macrophages and T-lymphocytes take part in the intimal arteritis of antibody-mediated rejection and accounted a highest percentage. But the T-lymphocytes which infiltrated under the endothelial cells were not only Cytotoxic T cell. NK cells can appear in the intimal arteritis of antibody mediated rejection. There was no similar report about these before. In glomerulitis, immunocytes infiltrated in the capillary were macrophages and neutrophil, this result was same to other people’s ideas.

Related Dissertations

  1. Therapeutic Effects of TACI-Ig on Systemic Lupus Erythematosus-like Mouse Model Induced by Active Chromatin Isolated from ConA-actived Lymphocytes,R593.241
  2. Cysteamine on T Lymphocyte Proliferation in Post-Operation Goats and Its Mechanism Underlying,S858.27
  3. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  4. The Clinical Significance of T Cell Subgroups and NK Cell’s Expression Level in Diffuse Large B-cell Lymphoma Patients,R733.1
  5. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  6. Effects of Geniposide and Its Metabolite Genipin on Human Peripheral Blood T Lymphocytes,R758.23
  7. Changes of Immunologic Function in Patients with Chronic Obstructive Pulmonary Disease and the Intervention by BCG Polysaccharides Nucleic Acid,R563.9
  8. The Effect of Mild Hypothermia Combined with TP-5 to T Lymphocyte Subsets and Prognosis of Patients with Severe Traumatic Brain Injur,R651.15
  9. Neutrophil / lymphocyte ratio in the preoperative diagnosis of uterine sarcoma application,R737.33
  10. Anti-tumor Effects of Lupeol at Different Regimens and Its Role in Immune Systems,R730.5
  11. The Change and Significance of CD4~+ CD25~+ T Regulatory Cells in Children with Primary Nephritic Syndrome,R726.91
  12. Establishment of Vero Cell Line Stably Expressing CDV SLAM Receptors of Different Animals,S855.3
  13. Research of Susceptibility Genes of Pediatric Diabetes Mellitus,R725.8
  14. The Detection of Anti-KINECTIN Antibody in Aplastic Anemia Patients’ Serum and Its Clinical Significance,R556.5
  15. The study of the mechanism of action of Guizhi Fuzi Decoction rheumatoid arthritis in rats,R285
  16. Effects of Ginseng Extracts and It’s Constituent on Immunological Function in Mice,R285.5
  17. Study on the Usage of T-Cell Receptor α and β Chains of the HBV-Specific CD8~+ T Lymphocytes in the Patients with Acute Hepatitis B,R392
  18. Experimental Study of Cell Immune and Intestinal Barrier After Severe Trauma - Hemorrhagic Shock in Rats,R641
  19. Experimental and Clinical Study of Combination Therapy with Microwave Ablation and Interleukin-2 for Primary Hepatic Carcinoma,R735.7
  20. Collection and analysis of breast milk on immune cells growth and development of preterm children,R722.6
  21. Integrative Medicine Immunity During Early Pregnancy Recurrent Abortion Retrospective Analysis,R714.21

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Urinary tract and male reproductive system surgery > Kidney surgery
© 2012 www.DissertationTopic.Net  Mobile