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The Expression Mechanism of Calmodulin in Rat with Brain Contusion

Author: HuangBoCong
Tutor: ZhangJian
School: Dalian Medical University
Course: Neurosurgery
Keywords: Traumatic brain injury Animal models Calcium overload Calmodulin
CLC: R651.1
Type: Master's thesis
Year: 2011
Downloads: 7
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Abstract


Objective: .1. establish a rat model of traumatic brain injury. 2. research brain injury and regional calcium calmodulin expression and variation.Methods: Use SPF level Sprague_Dawley (SD) rats of either sex, weighing 180-220g, from the Experimental Animal Center of Dalian Medical University. 72 rats were randomly divided into control group and the brain injury group, 36 rats in each group. Brain injury group by 0.5 m×800 g and 1 m×800 g against the weight of treatment, respectively, half an hour after injury, 8 hours and 120 hours for testing. The sham treatment control group, only the scalp incision for cranial bone window, not brain injury, and brain injury group, detected at the same time. Animals with brain injury with cylinder pads are made of diffuse brain injury complicated with focal brain injury models. Satisfied with brain injury after rat anesthesia, fixed in the foam pad, respectively, half an hour after injury, 8 hours and 5 days after magnetic resonance examination, comparison of dynamic MRI signal changes. And in animal feed to the required time, with 4% chloral hydrate (1ml/100g) after intraperitoneal injection of anesthesia, conventional perfusion fixation, the brains were removed intact. Brain tissue in 4% paraformaldehyde 12 hours after doing coronal slices, slice thickness 6μm, embedded in paraffin, immunofluorescence was used to detect calmodulin, and set the control analysis.Results: 1, by comparing the rat MRI T2, SWI images found to increase with the extent of brain injury, magnetic resonance image signals with significantly increased, 120 hours after brain injury in rats decreased MRI signal. 2, immunofluorescence results indicated that brain injury group in half an hour, 8 hours, 120 hours observation time 3 compared to both positive expression of calmodulin. Between the two groups found that brain injury group than in the control group, the expression of calmodulin, the difference was statistically significant (P <0.01) for half an hour after brain injury in rats found that expression of calmodulin started to increase after 8 hours Peak results from the immunofluorescence can be seen more intense positive expression of calmodulin, 8 hours after brain injury height of 0.5 meters and 1.0 meters in the brain injury group, the expression of calmodulin levels were 50.38±9.32 And 77.46±8.33, with a half-hour group and 120-hour group compared with the difference significant, (P <0.05). Then gradually decreased close to the control group. 3. Comparison of magnetic resonance imaging and immunofluorescence images of the dynamic changes of time and found good agreement between image changes.Conclusion: Free fall can be successfully used in the production of brain injury in animal models, can be used for a variety of diseases, brain injury Experimental Research. Calcium ion regulation of brain injury under the expression of calmodulin in rat brain damage and injury time-dependent. Brain damage more positive expression of TSP tune stronger after 8 hours after brain injury to reach peak expression gradually decreased after 120 hours. Calmodulin involved in the pathophysiology of brain injury and repair of the whole process.

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