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Influence of Transient Hypoxia on the Expression of NeuroD and Its Role in the Delayed Brain Neurogenesis

Author: LiuXiaoYan
Tutor: XuJianWen；WangZuo
School: Fujian Medical
Course: Neurobiology
Keywords: NeuroD Transient hypoxia Nerve regeneration
CLC: R714.7
Type: Master's thesis
Year: 2009
Downloads: 33
Quote: 0
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Abstract


Objective To observe the transient hypoxic rat brain endogenous differentiation factor (Neurogenic differentiation, NeuroD) changes in the expression levels and their relationship with the regeneration of the nervous system, to explore its possible role in the regeneration of the nervous system. NeuroD mRNA expression changes after transient hypoxia detected by RT-PCR. Then through in vitro cultured rat hippocampal neurons, RT-PCR was used to detect changes NeuroD mRNA expression levels in the cells after hypoxia of BrdU immunohistochemical detection of hypoxic cell proliferation; intrauterine to establish fetal rat delayed cesarean section The distress model animal model group neuroethology detect changes in situ hybridization to detect postoperative days hippocampus structure NeuroD mRNA expression levels. After the results of transient hypoxia NeuroD mRNA expression levels increased, and the expression of the constant increase with prolonged hypoxia time. The immunofluorescence showed, dispersed in the cerebral cortex and subcortical regions NeuroD spotty or cord-like distress NeuroD expression levels before and after no significant changes (P gt; 0.05). Relatively concentrated expression in the caudate putamen region NeuroD expression was significantly increased distress. Hippocampal neurons cultured in vitro 5d, hypoxia RT-PCR detected after 3h NeuroD mRNA expression was significantly increased BrdU immunohistochemistry of hypoxia culture BrdU-positive cells detected, that some neurons Back to cell cycle re proliferate. Body through delayed cesarean section and fetal distress model. The rats neuroethology test results show that the intrauterine distress the 10min group and the control group compared with no significant differences in motor coordination and learning ability of rats statistical model rats regain memory capacity significantly improved (P lt; 0.05). In situ hybridization showed that the model group in terms of the the hippocampus structure of dentate gyrus granule lower or CA1 region, NeuroD in P13, P20, expression levels of expression of P27 day control group has significantly higher compared to the most obvious, and P20 days. Conclusion 1. Demonstrated in vivo animal experiments NeuroD expression levels after transient hypoxia increased NeuroD expression level increased in vitro cultured hippocampal neurons after hypoxia, accompanying some neurons return to the cell cycle, re-separatist proliferation 3 hippocampal dentate gyrus granule lower and CA1 NeuroD expression changes with the nervous system regeneration showed high expression of NeuroD may be involved in the differentiation of neural stem cells, play a certain role in the nervous system regeneration process over the correlation

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