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Expression of P2X1,P2X3 Receptors and Their Role in Rat Bladders with OAB-symptom

Author: HeYuLin
Tutor: ShenJiZuo；LiuXiaoDong
School: Kunming Medical College
Course: Surgery
Keywords: Purinoceptor P2X receptors Bladder Overactive bladder symptoms Cystitis
CLC: R694~.5
Type: Master's thesis
Year: 2009
Downloads: 101
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Abstract


Objective: To study the P2X1 receptor and of P2X3 with OAB symptoms may be different causes symptoms of OAB on rat bladder P2X3 receptor expression and meaning, explore the purinergic receptor P2X1 relationship. Methods: Using different methods produced symptoms of OAB rat model as the experimental group and the control group of normal rats. The experimental group divided into five subgroups according to different causes: bladder outlet obstruction (BOO) 3 weeks associated with OAB symptoms rats group 5, bladder outflow obstruction group of six weeks with symptoms of OAB rats 6 bacterial cystitis caused by OAB symptoms rats group 5. The high off myeloid caused OAB symptoms in rats group 6, OAB symptoms in rats induced by cyclophosphamide group (n = 6). Each experimental group underwent urodynamic testing, confirmed OAB symptoms, take the rats bladder. Tissue stained by HE, Masson staining; P2X1 receptors, P2X3 receptor immune staining (SP method) process, and the results of immunohistochemistry by Image-Pro Plus6.0 software for semi-quantitative treatment calculated optical density (OD) value. The results were analyzed statistically using analysis of variance. Results: establish a rat model, BOO3 weeks of modeling 10 rats produces symptoms of OAB 5 accounted for only 50%, BOO6 weeks modeling 10 OAB symptoms in rats only 60% of bacterial cystitis made modulo 6, 1 death, 5 only accounts for 100% of rats produces symptoms of OAB OAB symptoms in rats only 100% the high off myeloid rat model 6, 6 cyclophosphamide induced cystitis modeling produce symptoms of OAB rats only 100%. Morphology, bladder outflow obstruction three weeks and six weeks marrow and high off the rat bladder smooth muscle cell hypertrophy, thickening of the cell muscularis; Cells gap widened, full of collagen fiber components. Bacterial cystitis, cyclophosphamide-induced cystitis rat bladder showed inflammatory changes of the bladder. Immunohistochemistry showed P2X1 receptor P2X3 receptors in the bladder on the normal control group, the experimental group were expressed, the expression levels of the experimental group was significantly higher than that in the control group (P <0.05), P2X1 receptor is mainly expressed in the detrusor P2X3 receptor expression in the bladder epithelium. There are also differences between the part of the group. Conclusion: The different causes changes in OAB symptoms rat bladder pathology different; OAB symptoms bladder P2X1, P2X3 receptor expression were significantly higher than in normal rats, which the P2X1 major expression in detrusor of P2X3 receptor expression in bladder epithelium, the amount of change of P2X1 and P2X3 receptor expression may be closely related and OAB symptoms, one of the common molecular biological mechanisms may be different causes of OAB symptoms; of P2X1, P2X3 receptors in OAB symptoms in molecular biology mechanism depth study of the possible to mention theoretical basis for the future development of the new mitigation clinical symptoms of overactive bladder drugs.

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CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Bladder disease
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