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[Objective: To study the role of urokinase-type plasminogen activator receptor (uPAR) in the process of maintaining normal sperm motility, viability, and participate in the sperm acrosome reaction, and to explore the underlying mechanism. 【Method】: collect 20 healthy men with fertility masturbation semen. Divided into two parts. The first part will be a final concentration of 10μg/ml, 50μg/ml, 100ug/ml anti-uPAR antibody were incubated in vitro with sperm upstream optimization, while a control group, 10min, 30min and 60min semen analysis. Second part of the normal fertile male sperm upstream optimize treatment induced sperm capacitation, the establishment of the normal control group, and then a final concentration of 10μg/ml, 50μg/ml, 100ug/ml anti-uPAR antibody with upstream optimized processing sperm co-incubated for 60 min induced sperm acrosome reaction, the pea lectin specific fluorescent markers - complexation with fluorescein isothiocyanate (FITC-PSA) Mark acrosome, by flow cytometry top the integrity of the body. [Results]: 1) The first part, after 10min incubation, sperm motility and viability of each group showed no significant difference (P gt; 0.05); the 10μg/ml antibody group compared with the control group, motility, viability, and there was no obvious difference (P gt; 0.05). 30min and 60min after incubation of 50μg/ml antibody group and 100ug/ml-antibody group compared with the control group a significant difference (P lt; 0.05), its motility, viability. With the rise of the anti-uPAR antibody concentration and the extension of the incubation time, sperm motility showed a downward trend. 2) The second part, the final concentration of 10μg/ml, 50μg/ml and 100ug/ml anti-uPAR antibody group, the acrosome marked fluorescence values ??were significantly higher than that in the control group, the difference was significant (P lt; 0.01) . 100μg/ml fluorescence values ??higher than the previous two experimental groups, but the the fluorescence value comparison between the three experimental groups, the results were no significant differences (P> 0.05). [Conclusion]: 1, different concentrations of anti-uPAR antibody in vitro can significantly reduce the vitality of the sperm motility rate and sperm motility function. 2, high concentrations of anti-uPAR antibody can inhibit the in vitro sperm acrosome reaction occurs. 3, uPA / uPAR affect sperm motility and acrosome reaction mechanism may be an increase in free calcium concentration in the sperm cells. 4, anti-uPAR antibody in vitro, these reactions are likely through assisted reproductive technology and effective use of scientific research and clinical treatment of infertility.
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