Dissertation > Excellent graduate degree dissertation topics show

The Changes of GAP-43 and CGRP Expression in the Spinal Cord after the Spinal Cord Injured in Rats.

Author: FanShouCheng
Tutor: YangZhiYong；WangTingHua；DingPeng
School: Kunming Medical College
Course: Surgery
Keywords: the spinal cord injury CGRP GAP43 rat
CLC: R651.2
Type: Master's thesis
Year: 2009
Downloads: 74
Quote: 0
Read: Download Dissertation

Abstract


Objective Establishing cryogenic spinal cord injury rat model,in order to observe the changes of GAP-43 and CGRP expression in the spinal cord,and study the primary mechanism on the spinal cord injury.It will provide theoretical evidence for the experimentally therapy of the impaired spinal cord.Methods Sixty adult Sprague-Dawley rats were randomly divided into normal group,sham-operated group and the spinal cord injury group.In the experiment group,after the T9-T11 spinous process and the corresponding vertebral lamina were removed,the spinal cord of T10 segment was freezed by liquid nitrogen.In sham-operated group,rats received only T10 laminectomies. In normal group,rats not received opreration.The normal group and sham-operated group rats were killed at 14d after operation.The experimental group rats were killed at 2h,6h,12h,24h,48h,3d,7d and 14d after operation according to the survival time.The expression of GAP-43 and CGRP in the spinal cord detected by immunohistochemical staining and image analysis methods.Results The hindered walking and equilibrium function were evident to show that the cryogenic spinal cord injury rats model is successeful.The expression of GAP-43 in the uninjured spinal cord was lower.But the expression of GAP-43 was increased in the spinal cord after the spinal cord was freezed by liquid nitrogen.The expression of GAP-43 in the spinal cord after injury was increased at day 3,and further increased to the highest expression at day 7,but the expression of GAP-43 was reduced quickly atday 14.The expression of CGRP in the uninjured spinal cord was high.But the expression of GAP-43 in the spinal cord after injury decreased at day 3,and further reduced at day 7 and 14.Conclusion The cryogenic spinal cord injury rat model is a stable and reliable spinal cord injury animal model.The expression of GAP-43 and CGRP were changed in the spinal cord after the spinal cord injured in rats.It suggested that GAP-43 and CGRP may participate in the process of degeneration and regeneration after nerve injury and promote nerve regeneration.But the primary mechanism on the recovery of spinal cord injury need to study in future.

Related Dissertations

  1. The Effect of Tanshinone ⅡA and Niacin on the Cardiovascular Function of Infant Obese Rat,R285.5
  2. Therapeutical Effects of Diammonium Glycyrrhizinate Lipid Ligand on Nonalcoholic Steatohepatitis and Its Partly Mechanisms of Action,R575.5
  3. Dynamic Effect of Cold Restraint Stress on Damage of Ultrastructure and Expression of Heat Shock Protein in Rat Tissues,S858.91
  4. Anesthesia Management of Cervical Spinal Cord Injury,R614
  5. Experiment Study of Ethylhexyl Phthalate (DEHP) and It’s Metabol-ites (Single-ethylhexyl Phthalate, MEHP) Influencingon Sper Matogoniain Young Male Wistar Rats,R329
  6. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  7. The Regulatory Role of Proliferation and Apoptosis of ERK1/2 and JNK Signaling Pathways in Eight Liver Cell Types during Rat Liver Regeneration,Q418
  8. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  9. Effects of High-salt Dieet on Blood Pressure and the Remodeling of Carotid Arteries and the Intervention of Telmisartan in Wistar Rats,R544.1
  10. The Espression of C-reactive Protein in Rats of the Chronic Obstructive Pulmonary Disease with Diabetic,R563.9
  11. Relationship and Significance of Gene Transcription Profiles in Rat Liver Regeneration and Non-Alcoholic Fatty Liver Disease Occurrence,R575.5
  12. The Expression of Visfatin in Liver of Rats in Different Glucose Metabolic Statuses and Effects to Glucose and Energy Metabolism,R587.1
  13. The Beneficial Effects of Soy Isoflavones in Osteoporosis of Ovariectomized Rats,R580
  14. Experimental Investigation of Grafting Pathways of Bone Marrow Stromal Cells into Spinal Cord Injury in Rats.,R651.2
  15. 3 T Magnetic Resonance Diffusion Tensor Imaging and Fiber Tracking in Detecting Acute Spinal Cord Injury,R651.2
  16. Morphological Characteristics of Brain Capillary Endothelial Cells and Neurons in a Ticellular System for Study on Neuroprotection and Neuroregeneration in Vitro,R651.1
  17. The Study of the Influence of BMS-345541 on Motor Function of Hind Limbs and the Infiltrating Leukocytes after Spinal Cord Injury in Rats,R651.2
  18. Influences of Temperature on SCF/c-kit Prolifertation Pathway and Cell Cycle of Rat Spermatogonial Cells Cultured in Vitro,R698
  19. The Mechanism of CREG Regulated Neuronal Apoptosis in Rat Intracerebral Hemorrhage,R743.34
  20. Rat liver regeneration and acute liver failure occurring gene transcriptional profiling studies and their significance,Q418
  21. Kangfuxin treating rats with experimental colitis efficacy and mechanism of,R285.5

CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
© 2012 www.DissertationTopic.Net  Mobile