|
Objective: To build the Sprague-Dawley (SD) rats with moderate spinal cord injury model, combined with the BBB behavioral score to explore estrogen (Estrogen) from the level of oxidative stress (Oxidative stress) for acute spinal cord injury (Acute spinal cord injury, ASCI) therapeutic effect and mechanism of neuroprotection. Methods: 65 adult male SD rats were randomly divided into three groups: sham group, damage, injury after estrogen treatment group, 6h, 1d, 3d, 5d, 7d five time points in each group is divided into five alkylene group. Each point of each of the sham group 3. Each point of the injury and treatment groups of five each, ASCI Allen's combat caused by spinal cord 10 thoracic (T10). Given intramuscular injection of estrogen treatment group after 15min 4mg/kg injury group at the same time to give a volume of 0.9% sodium chloride solution (NS) intramuscularly. After 1d, 3d, 5d and 7d BBB behavior score was judged rats limb motor function recovery; injury site for the center and at five time points after drawn chemical colorimetric determination of super oxide vitality of superoxide dismutase (SOD), nitric oxide synthase (TNOS and iNOS), hydrogen peroxide (H 2 O 2 ) content, as well as anti-hydroxy freedom base (OH) capacity and resistance to superoxide anion (O 2 - ) capacity. Results: Postoperative 1d, 3d, 5d, 7d BBB score of the treatment group was significantly higher than the injury group (P <0.05); SOD activity value of postoperative 1d, 3d, 5d treatment group was significantly higher than the injury group (P <0.05). and reached a peak in 5d injury group SOD activity value was significantly higher (P <0.05) after 7d; the the postoperative the 5d therapy group and control group TNOS were significantly increased, compared with the sham group was statistically different (P <0.05), but between the two groups showed no difference (P> 0.05), 7d surgery treatment group compared with the control group TNOS was significantly decreased (P <0.05), but still higher than the sham group (P <0.05); 5d after the treatment and control groups iNOS was significantly increased compared with sham group, but between the two groups showed no difference (P> 0.05), the treatment group iNOS 7d rapid decline in the control group iNOS remained elevated, two groups significant differences (P <0.05); the postoperative the 1d therapy group anti O 2 - capacity value increased compared with the control group (P <0.05), but lower compared to the sham group the control group (P <0.05), 7d anti-O 2 - capacity value compared with the treatment group and the sham group was significantly higher (P <0.05), the treatment group and the sham no difference in the surgical group; postoperative 1d, 3d, 5d, 7d treatment group H 2 O 2 levels were significantly higher than that in the control group (P <0.05) and 1d The two peaks 5d 7d recovery to the level of the sham control group 7d still remains low; postoperative 6h, 1d, 5d treatment group anti-OH capacity value compared with the control group, a significant difference (P <0.05) treatment group reached the peak, 5d control group reached the peak at 1d. Conclusion: The early high-dose estrogen therapy to promote SD rats ASCI recovery of neurological function; early after spinal cord injury that increased production of reactive oxygen species (Reactive oxygen species, ROS), oxidative stress involved in the pathophysiology of secondary injury process; compensatory increase in antioxidant system, but compensatory response appeared later decompensation limited extent, estrogen therapy can advance and amplify this compensatory response; the NOS impact of estrogen therapy on the occurrence of late without too obvious; vigor or ability of some members of the antioxidant system values ??appear to change as well as the distribution of inconsistency may be due to ROS generated transformation path itself is a time, with the participation of multi-component process; estrogen may indirectly by improving the capacity of the antioxidant system reduce the generation of ROS, reduce secondary spinal cord injury.
|