Dissertation > Excellent graduate degree dissertation topics show

Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol

Author: WeiZuo
Tutor: YuSong
School: Zunyi Medical College,
Course: Pediatric surgery
Keywords: Hemangioma Vascular endothelial cells Cell culture Propranolol Vascular endothelial growth factor Matrix metalloproteinases-2
CLC: R739.5
Type: Master's thesis
Year: 2011
Downloads: 5
Quote: 0
Read: Download Dissertation


Objective To observe the influence of VEGF and MMP-2 of hemangioma endothelial cells in vitro culture by propranolol. To study the mechanism of the hemangioma’s treatment by propranolol.Methods The hemangioma vascular endothelial cells which came from the specimens of infant hemangioma were cultured by explant combined with trypsin-digested technique.The expression of the factorⅧrelated antigen which only existed in endothelial cells to identify the hemangioma vascular endothelial cell were studied by the immunohistochemistry technique. When the cells were in the logarithmic phase,the culture medium was exchanged to serum-free medium to make the cell synchronization in 48 hours. Propranolol was used on cells at three different concentrationgs which were 0μmol/L,0.5μmol/L and 1.0μmol/L. Vascular endothelial growth factor(VEGF) and Matrix metalloproteinases-2(MMP-2) were detected by RT-PCR when the cells were cultured after 24 hours,while cell apoptosis were investigated by flow cytometry technique,and then the apoptosis related of VEGF and MMP-2 in relative mRNA expression level of vascular endothelial cells was analyzed.Results1.The hemangioma endothelial cells emigrated from the tissue began to grow fast and covered the bottom of culture bottles after three or four weeks. The expression factorⅦassociated antigen was positive in the endothelial cells in vitro culture.2. Compared with the apoptosis rate of hemangioma endothelial cells which were cultured after 24 hours, both of two propranolol treatment groups were significantly higher than the control group(P<0.01).The mRNA relative expression of VEGF was 1.02±0.42 in the group of 0.5μmol/L propranolol and 0.93±0.22 in the group of 1.0umol/L propranolol,both of them were lower than the control group which was 1.61±0.52(P<0.05). The relative expression of MMP-2 was 0.77±0.18 in the group of 0.5μmol/L propranolol and 0.67±0.27 in the group of 1.Oμmol/L propranolol,both of them were lower than the control group which was 1.47±0.57(P<0.05). Both of VEGF and MMP-2,compared with the 0.5μmol/L group, the difference was not statistically significant was found in 1.0μmol/L group(P>0.05).Conclusion Propranolol may promote the apoptosis or inhibit the growth of hemangioma vascular endothelial cells in vitro culture, and the mechanism might contribute to the reducing of mRNA expression of VEGF and MMP-2.

Related Dissertations

  1. Effects of Fix 6,7-furocoumarins on the Hepatic Microsome Cytochrome P2C9, 2C19 Activity in Rats,R965
  2. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  3. Identification of Biological Characteristics with Human Keloid Fibroblast-like Mesenchymal Stem Cells,R622
  4. In Vitro Culture and Identification of Vascular Smooth Muscle Cells from Rat Intra- and Extracranial Arteries,R743.3
  5. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  6. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  7. Primary Research on Strring Culture and Storing of Human Dermal Fibroblasts,R329
  8. Discuss of the Difference between Regular Hepatectomy and Enucleation of Liver Hemangioma,R735.7
  9. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  10. A Simple and Efficient Method That Uses Low Concentration Fetal Bovine Serum to Culture and Purify Schwann Cells,R329
  11. Expression of TNF-α, MMP-9 and VEGF in BALF of Rats with Emphysema and Their Mutual Relationship,R563.3
  12. The Investigation of Relationship between Therapeutic Effect and Prognosis and Changes of Peripheral Blood Vascular Endothelial Growth Factor and Metallothionein in Patients with Advanced Lung Cancer,R734.2
  13. Study on the Correlation btween Serum, Aqueous Humor VEGF, IL-6 Levels and the Neovascular Glaucoma,R775
  14. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  15. Vascular endothelial growth factor on endothelial cells concentration of magnesium ions,R965
  16. Osteopontin through the MAPK signaling pathway and increased MMP9 expression correlation with embryo implantation,R321
  17. Fibroin microspheres and their use as cell carriers in study,R318.08
  18. Effect of Different Glucose Concentrations on Autophagy in Neonatal Rat Cardiomyocyte Culture,R542.2
  19. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  20. Meningiomas and MMP-9, VEGF expression and tumor angiogenesis and recurrence,R739.45

CLC: > Medicine, health > Oncology > Skin tumors
© 2012 www.DissertationTopic.Net  Mobile