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The thesis is divided into three parts: a rat orthotopic left lung transplantation model; nitric oxide synthase expression changes during ischemia and reperfusion in rat lung transplantation;, L-arginine and aminoguanidine in rat lung transplant ischemia-reperfusion injury in rats. A rat orthotopic left lung transplantation model to investigate on the basis of the \foundation; methods under a microscope using three sleeves anastomosis complete rat orthotopic left lung transplantation model, the feasibility of the success rate of surgery, duration of surgery, the transplanted lung function, pathological changes, and imaging examination assessment model; The results completed rat orthotopic left lung transplantation in 20 cases, the surgical success rate of 85%. For lung lavage to obtain completion time 13.1min ± 0.9min, for lung vitro casing time 9.3min ± 0.7min, for receptor action veins and bronchial anastomosis time 33.7min ± 1.7min. The occlusion experiments show the transplanted lung single-lung ventilation to maintain more than 20min. Pathology transplanted lung reperfusion 2h able to copy typical ischemic-reperfusion injury model; Conclusion In this study, on the basis of the three sleeves anastomosis, home and abroad, the advantages and disadvantages of the rat lung transplantation model, according to the research needs of appropriate improvements, trying to establish the best model of the left lung transplantation in rats. Completion of the single model under a microscope, shortened casing and dynamic venous and bronchial anastomosis time, reduce warm ischemia time, improve the success rate of surgery and rat survival. Pathology confirmed the model can replicate the typical transplant lung ischemia-reperfusion injury pathological changes, suitable to the ideal model of ischemia-reperfusion injury. Nitric oxide synthase in rat lung transplantation ischemia-reperfusion Objective To observe the nitric oxide synthase (NOS) expression changes in the course of ischemia-reperfusion expression changes in rat lung transplantation; method 12 SD rats completed 6 rat left lung transplant, respectively thoracotomy (I group) after lavage (II group) and cold ischemia (III group) to take for rat right lung tissue RNA was extracted, and then 2h (Ⅳ group) taken after perfusion donor left lung tissue RNA was extracted, the reperfusion after 2h take the right lung tissue of the receptor (Ⅴ group) RNA was extracted, reverse transcriptase to generate of cDNA. Application of fluorescence quantitative PCR detection of lung tissue in the endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) expression; Results Compared with group Ⅰ, group Ⅱ, group Ⅲ and Ⅴ group eNOS and iNOS no significant change. Ⅳ group Ⅰ compared eNOS expression was significantly reduced, and iNOS expression was significantly increased; Conclusion lung transplant ischemia reperfusion observation of the various stages of the process, the importance of nitric oxide synthase changes cause the transplanted lung confirmed reperfusion one of the factors. L-arginine and aminoguanidine in rat lung transplant ischemia-reperfusion injury in rats purpose to explore the L-arginine and aminoguanidine ischemia-reperfusion injury in rats and its mechanism in rat lung transplantation ; Methods 48 SD rats were randomly divided into four groups, lung transplantation group (A), L-arginine group (B group), aminoguanidine group (C), and L-arginine and aminoguanidine group (D). N = 12, line allograft left lung transplantation. After the success of the operation intraperitoneal injection of saline, L-arginine, aminoguanidine and L-arginine and aminoguanidine. Take the top of the transplanted lung lung tissue after reperfusion 2h detection of myeloperoxidase (MPO) activity, malondialdehyde (MDA), superoxide dismutase vitality (SOD), endothelial nitric oxide synthase (eNOS) and Determination of inducible nitric oxide synthase (iNOS) activity; venous blood Determination of NO content in the blood; take the lower end of the transplanted lung lung tissue wet and dry weight ratio (W / D), respectively pathological morphology of the lung tissue specimens of four groups ; result reperfusion after 2h, compared with group A, group B iNOS and eNOS activity increased NO content in the blood than in group A, the transplanted lung W / D, MPO and MDA content decreased SOD levels increased ( P <0.05). INOS activity was reduced in group C (P <0.05), no change in eNOS activity and NO content is lower than in group A (P <0.05), no significant change in the W / D, SOP, MPO and MDA. Group D of iNOS activity decreased (P <0.05), eNOS activity increased (P <0.05)) NO content in the blood increased (P <0.05), W / D, of MPO, MDA were lower (P <0.05), SOD increased (P <0.05). Group B and Group D, D group iNOS activity reduce (P <0.05), while no change in eNOS activity, NO content lower (P <0.05), its W / D, MPO, MDA decreased (P <0.05) , SOD increased (P <0.05). The pathomorphological examination showed Group D inflammatory cell infiltration and inflammatory exudation lightest, followed by group B, group A and group C the worst; conclusion transplant reperfusion early use of L-arginine can reduce the transplanted lung ischemia reperfusion injury, the combination of L-arginine and aminoguanidine early reperfusion application aminoguanidine reduce lung ischemia-reperfusion injury in the simple application of L-arginine, but did not lessen the damage of the transplanted lung. Application of L-arginine attenuates ischemia reperfusion injury related to the increase of NO produced by eNOS pathways.
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