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Purpose: pure low-temperature preservation standing heart preservation effect has been verified, but the early postoperative cardiac function suppression is quite common, and the heart of this method to save time is shorter, only 4-6h. Continuous low flow perfusion is a new type of heart preservation methods, this project is using a modified Langendorff device for heart preservation solution HTK solution and FWM liquid, relatively simple low temperature was allowed to stand to save two different preservation methods and continuous low-flow perfusion in vitro The effect of the preservation of the rat heart, myocardial preservation and HTK solution and FWM liquid evaluate the effect. Methods: 32 male SD rats were randomly divided into four groups (n = 8), The HTK solution pure hypothermia stand for group (H1 group), HTK solution continuous low flow perfusion group (H2 group) FWM solution simply stand for group ( F1 group), FWM liquid continuous low flow perfusion group (F2 group). Thoracotomy hearts were removed quickly transferred and fixed in the Langendorff perfusion apparatus, constant pressure the KH solution by aortic perfusion 30min, 4 ° C preservation solution via the aortic root perfusion, F1, F2 group perfusion FWM liquid, H1, H2 group perfusion HTK solution, to be cardiac arrest at 4 ℃, F1, H1 group heart were immersed in the FWM liquid and HTK solution, standing save 8h; F2, H2 group heart 10cm H2O pressure infusion FWM liquid HTK solution perfusion 8 h. 8h after re-move the mouse heart Langendorff apparatus reperfusion KH solution for 60min. In irrigation stopped before and after reperfusion measured by heart rate (HR), coronary flow (CF), left ventricular developed pressure (LVDP), heart rate indoor pressure the multiplied (RPP), the maximum rate of rise of left ventricular pressure (dp / dtmax) indicators of left ventricular pressure maximum decreasing rate (-dp/dtmax) on the recovery rate and calculate the various parameters while sacrificing coronary effluent for the determination of cardiac enzymes (CK-MB, LDH) leakage. Take myocardial tissue determining moisture content produced by light microscopy specimens observed structural changes of myocardial tissue perfusion after. Results: After reperfusion, none of the F1 group of 8-cardiac resuscitation cardiac function recovery rate of 0. We found the process of reperfusion the heart pale color, touch the hard, such as \Myocardial water content, cardiac enzyme leakage was significantly higher than the other three groups (P lt; 0.01). H2 group after reperfusion left ventricular developed pressure recovery rate (77.7 ± 3.4)%, heart rate recovery rate (80.2 ± 5.3)%, the recovery rate of coronary flow (54.6 ± 6)%, heart rate indoor pressure recovery rate (60.6 ± 2.1) %, myocardial water content (79.5 ± 0.7)%, and dp / dtmax (60.1 ± 5.9)%, -dp/dtmax (57.6 ± 4.6)% compared with the other three groups, the difference was statistically significant (P lt; 0.05) myocardial protective effect. Light microscope the pathological picture show: H2: interstitial mild edema, myocardial arrangement rules occasionally myocardial cells rupture, dissolution, addicted to color. F1: interstitial edema, large areas of myocardial cell degeneration, disorganized, and the stripes disappear, rupture of the myocardial cells, dissolved addicted to color. Group H2 and F2 group, the H1 groups and the F1 group compared save the same technology and save time, FWM HTK solution myocardial protection is better than liquid, and the difference was statistically significant (P lt; 0.05). Conclusion: The experimental results showed that: put it aside with a simple low-temperature preservation techniques for low-flow perfusion myocardial protective effect is stronger, prolong cardiac Save time; under the same experimental conditions, the HTK solution for myocardial protection better than the FWM liquid.
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