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Research background osteoporosis (osteoporosis, OP) is based on a reduction of bone mass , the fine structure of the bone tissue destruction lead to increased bone fragility and increased risk of fracture is characterized by a systemic systemic bone disease in the elderly and postmenopausal women. Osteoarthritis (osteoarthritis, OA) in cartilage change as the main feature , which causes the subchondral bone necrosis , cystic degeneration , bone density increased and a non-specific inflammation associated with osteophyte formation . OP and OA is a common disease of the elderly musculoskeletal system , and is often associated with each other , there is a certain relationship between the two . Diphosphonates (bisphostates, BPs) pyrophosphates stable analogues, in vitro dose - dependent inhibition of hydroxyapatite crystal growth, in vivo inhibition of ectopic calcification , can inhibit a variety of drug-induced bone resorption . Foreign stories BPs can reduce the degradation of type II collagen and inhibit apoptosis of chondrocytes , has a protective effect on cartilage ; alendronate (alendronate sodium) is three generations of two bisphosphonates , clinical treatment of osteoporosis commonly used drug , its role in cartilage no study reported . Objective To evaluate the alendronate rat articular cartilage in the treatment of osteoporosis . Select the 36 -month-old SD rats were randomly divided into three groups ( n = 12 ) : alendronate group (OVX ALEN) and saline group ovariectomized (OVX NS) ; sham group ( SHAM) only surgery , without ovariectomy , where OVX ALEN group given alendronate solution ( 3 mg / ml ) 36mg/kg/w dose gavage in OVX NS group and SHAM group was given the same dose of 0.9% saline gavage take the rat proximal tibia , 4, 8 and 12 weeks after administration line HE , toluidine blue staining ; femur distal row HE AB -PAS ( periodic acid Schiff ) Alcian blue staining and measurement of cartilage thickness ; immunohistochemical staining of collagen type II , the slice image analyzer for measuring the average gray value . 4W when cartilage thickness in each group , the number of chondrocytes similar , four kinds staining did not change significantly ; 8,12 W OVX NS group cartilage thickness ( P <0.05 ) compared with the the OVX ALEN group and SHAM group ; 12W OVX NS group immunohistochemical staining of collagen type II gray value lower than the the OVX ALEN group and SHAM group ( P <0.05 ) . Articular cartilage structure in case of conclusion osteoporosis , metabolic changes taking alendronate has a protective effect on articular cartilage .
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