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Experimental Study of Huaier-induced Apoptosis in Mouse Transplanted Tumor Cell of Liver Cancer

Author: LiuXueJun
Tutor: LingChangQuan
School: Second Military Medical University
Course: Traditional Chinese Medicine
Keywords: Hepatic carcinoma Huaier cream Induction of apoptosis Bcl-2 Bax
CLC: R735.7
Type: Master's thesis
Year: 2009
Downloads: 127
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Abstract

Background primary liver cancer (PLC) has become our second malignancies etiology, is one of the most common malignant tumor. The statistics show that the world's annual new cases of liver cancer about 45% in China's mainland. Liver cancer mortality rates in China increased 41.7 percent to nearly 20 years, the trend can be seen the high incidence of liver cancer in China has been very severe [1]. The study found that Chinese medicine with a variety of Western conventional treatment of advanced primary liver cancer, with significant improvement in clinical symptoms and signs, and improve the quality of life and extended survival period [2]. In recent years, the development of traditional Chinese medicine (TCM), purified visible components screened from traditional Chinese medicine, such as herbal polysaccharide, antitumor experiments, made some progress to provide a way for anti-cancer Chinese medicine. The Huaier a medicinal fungus growth in the aging locust tree dry - Cassia Trametes (Trametes robiniophila Murr), its anticancer constituents of proteoglycans (PS-T). Now its finished the - Huaier particles (national class of drugs) used clinically. Comprehensive modern literature of Trametes particles in clinical practice has been widely used in the treatment of a variety of tumors, and achieved a certain effect: a digestive system tumors: Huaier combined with conventional chemotherapy, can significantly improve gastric cancer after 5 years survival [3]; Huaier with chemotherapy can improve the immune function of patients with colorectal cancer, and to reduce the toxicity of chemotherapy, improve hematopoietic function [4]; 2, breathing system tumors: Huaier to significantly improve the immunity of non-small cell lung cancer patients efficacy. In explaining its anti-tumor mechanism, there are a large number of experimental studies. role. [Objective] 1, observed Huaier cream inhibitory effect on the growth of human hepatoma SMMC-7721 cell lines in vitro; observed Huaier cream inhibition of liver cancer in mice transplanted tumor cells and preliminary study its mechanism of action. [Methods] MTT assay Huaier-cream inhibition rate of liver cancer cells in vitro SMMC-7721 cell lines; observe Huaier cream mouse xenograft inhibition and induction of apoptosis subcutaneous injection of H22 mouse hepatoma cells, making the model of tumor-bearing mice, divided into group A (control group), B group (5-Fu group), C group (Huaier cream group), Group D (5 Huaier cream -Fu group), after the end of treatment, called tumor weight inhibitory rate was calculated; HE staining, the tumor tissue was observed pathological changes; TUNEL assay of tumor tissue apoptosis rate; using immunohistochemical methods to detect transplanted tumor cells organizations related apoptotic protein Bcl-2, Bax expression, to explore the possible mechanism of its induction of apoptosis. [Results] 1, the inhibition of cell proliferation in vitro experiments: Huaier cream of SMMC-7721 cells significantly inhibited, and the concentration increased with dose - response relationship, 10 mg / ml Huaier cream group 5 mg / ml Huaier clear paste group 48 h inhibition rate reached 65% and 55%, respectively. 2, in vivo inhibition of tumor growth experiment: A group (control group) in tumor-bearing mice transplanted tumor weight was significantly greater than in group B (5-Fu group), C group (Huaier cream group) and Group D (Huaier cream 5-Fu group) (P lt; 0.05, P lt; 0.05, P lt; 0.05), B, C, D group tumor inhibition rates were 31.2%, 27.1%, 47.5%, suggesting Huaier cream in vivo inhibit tumor growth; Group D tumor inhibition rate was significantly higher than B and C group, the difference was statistically significant (P lt; 0.05), prompted Huaier cream combined with 5-Fu medication can improve 5-Fu inhibit tumor effect. 3, the detection rate of apoptosis of tumor tissue by TUNEL method showed that apoptosis rate in order were: Huaier cream combined with 5-Fu group, the cream group of Huaier ,5-Fu group, the control group, the difference was statistically significant (P lt; 0.05). 4 related apoptotic protein Bcl-2 in the determination of the results of immunohistochemistry: control group, Bcl-2 positive expression rate was 43.02 ± 2.05%, significantly higher than the 5-Fu group, Huaier cream group, Huaier The positive expression rate of the cream combined with 5-Fu group, the difference was statistically significant (P lt; 0.05), indicating that Huaier cream can down-regulate the expression of tumor-bearing mice tumor tissue Bcl-2 protein. Related apoptotic protein Bax Determination results of immunohistochemistry: Huaier cream combined with 5-Fu group of Bax positive expression rate of 87.88 ± 9.72%, higher than that of the control group ,5-Fu group, Huaier cream group the difference was statistically significant (P lt; 0.05); clear cream of control group Huaier of group difference was not statistically significance (P gt; 0.05) shows that Huaier cream combined with 5-Fu can enhance the expression of Bax. [Conclusion] Huaier cream of SMMC-7721 cells in vitro inhibition of growth; 2 Huaier cream in the body by inducing apoptosis of mice tumor growth; 3, Trametes clear paste can down-regulate Bcl-2 gene protein expression, combined with 5-Fu obvious that raised the role of Bax in Huaier cream may be through the induction of tumor-bearing mice transplanted tumor tissue apoptosis, inhibition of tumor growth.

Experimental Study of Huaier-induced Apoptosis in Mouse Transplanted Tumor Cell of Liver Cancer

Abstract

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