Dissertation > Excellent graduate degree dissertation topics show

The Research of the Mechanism of Integron Mediated Drug-resistance in ESBLs-producing Strains

Author: ChenTi
Tutor: WuYong
School: Central South University
Course: Clinical Laboratory Science
Keywords: extended spectrumβ-lactamase integron multidrug resistance reverse PCR
CLC: R378
Type: Master's thesis
Year: 2011
Downloads: 74
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveTo identify the categories and gene cassettes arrangements of integrons, and their distributions in extended spectrum beta-lactamases (ESBLs)-producing E.coli and K. pneumoniae and investigate integron-mediated multidrug resistance of ESBLs-producing strains in clinic. The flanking sequences of classⅠintegron were investigated using reverse PCR.MethodsFrom June 2009 to December 2009,100 strains of extended spectrum beta-lactamases (ESBLs)-producing E.coli and K. pneumoniae were isolated from Third Xiangya Hospital of Central South University. PCR was used to detect the presence of integrons and the genes coding for blaTEM, blaSHV and blaCTX-M, amplify the variable region, PCR-RFLP to screen the homology of integron variable region, and the gene cassettes were detected by DNA sequencing. Genomic DNA was digested by HindⅢrestriction enzyme and using reverse PCR investigated the flanking sequences of classⅠintegron.ResultsThe expression of classⅠintegron that was found among ESBLs-producing strains was 69%. Comparing to those without classⅠintegron expression, the strains with classⅠintegron presented significant higher resistance to 12 antibiotics (P＜0.05), including cephalosporin, quinolones, sulfonamides andβ-lactam/enzyme inhibitors. In the genes that coding for blaSHV, blaTEM and blaCTX-M,, the expressions of classⅠintegron were 84.4%,72.9% and 68.9%. Reverse PCR amplified 1800bp size class I fragment of integrase gene flanking sequences. ConclusionsThere was a good correlation between integron-carrying and the ESBLs-producing of E. coli and K. pneumoniae strains. Distribution of classⅠintegron in extend-spectrum beta-lactamases (ESBLs)-producing strains is prevalent. Class 1 integron harboring multi-drug resistance has been identified among E. coli and K. pneumoniae isolated. Class 1 integron has taken important part in multi-drug resistance. The results stressed the need for surveillance of the integron content of nosocomial E. coli and K. pneumoniae populations may be critical to predict and prevent the spread of particular antibiotic resistance determinants.

Related Dissertations

  1. The Correlation Between Drug Resistance and Some Drug Resistance Genes in Sixty-one Gallibacterium Anatis Isolates,S852.61
  2. Screen Genes Affected the Activity of Quorum Sensing Regulator VqmA and Functional Analysis of LysR and MFS Proteins in V.choleare,R378
  3. Effects of SILA409 on the Expression of GRP78 in Nci-h446 Human Small Cell Lung Cancer Cells and Its Correlation with Chemotherapy Resistance to VP-16,R734.2
  4. Effect of GCS on Expression of MDR1mRNA and P-gp Function in K562/AO2 Cell Line,R733.7
  5. Establishment of Cisplatin- Resistant Gastric Cancer Cell SGC7901/DDP and the Effect of Recombinant Adenovirus Mediated Human IL-24 Gene to Chemosensitivity,R735.2
  6. Jieduhuayu party of the leukemia in K562/A02 HL60/ADR cell resistance reversal role,R733.7
  7. Expression and Significance of Breast Cancer Resistance Protein and Topoisomerase Ⅱ in Nude Mice Transplanted Tumor Model of A549/CDDP Multidrug Resistance Cell,R730.5
  8. Aminoglycoside-resistant Pseudomonas Aeruginosa and the Molecular Mechanism of Drug Resistance,R378
  9. Effect of CYP3AP1 and ABCC2 Gene Polymorphism on Lipid-lowering Efficacy of Atorvastatin,R589.2
  10. Surveillance of Antimicrobial Resistance and Detection of Integron Gene Cassettes among Escherichia Coli Isolates from the Intestinal of Animals,S852.61
  11. Investigation and Diversity Analysis of the Antibiotic Resistant Integron-bearing Bacteria in Aquatic Environment,Q938
  12. Integrons Detected by Multiplex PCR and Their Effects on Antibiotic-resistance of Staphylococcus Aureus,R440
  13. Research on Drug Resistant Genes of Plasmids in Multi-drug Resistant Acinetobacter Baumannii and Infective Chinese Herbals,R446.5
  14. Study on the Carbapenemases and Integrons among Carbapenem-resistant Acinetobacter Baumannii,R446.5
  15. Acinetobacter baumannii double ring of the aminoglycoside resistance phenomenon,R446.5
  16. Study of Drug-resistance Mechanism in Multi-drug Resistant Pseudomonas Aeruginosa,R446.5
  17. Study on Class Ⅰ Integron and ISCR1 in Non-Fermenting Gram-negative Bacilli from Clinical Specimens,R440
  18. Evaluation of Different Methods for Vibrio Parahaemolyticus Detection and Study on Characteristics of Genes in Environmental Strains,R155.5
  19. Gene Location of Class Ⅰ Integron Gene Cassette of Escherichia Coli from Porcine Normal Flora and Effects of Some Physical and Chemical Factors on Their Antibiotic Plasmids Curing,S852.61
  20. Research on β-lactam-resistance Gene in Lactose-fermentation Enterobacteriaceae in the Wastewater Treatment Plant,X172
  21. Study on the VEB-1 Gene Cassette in Clinical Pan-resistant Pseudomonas Aeruginosa and the Impact of Its Structure on the Integration of Sub-frequency Capture,R378

CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Pathogenic bacteria
© 2012 www.DissertationTopic.Net  Mobile