Dissertation > Excellent graduate degree dissertation topics show

Coagulation factor Ⅲ b in zebrafish embryonic development role

Author: ZhouRuiFang
Tutor: YuMin
School: Fudan University
Course: Molecular Medicine
Keywords: tissue factor vasculature brain development zebrafish
CLC: Q954.4
Type: Master's thesis
Year: 2011
Downloads: 64
Quote: 0
Read: Download Dissertation

Abstract


Tissue factor (coagulation factorⅢ) is a cell surface receptor binding to coagulation factorⅦ/Ⅶa and was initially recognized as an initiator of the extrinsic coagulation path way.The human TF gene has been cloned and is localized to chromosome 1 (p21-p22). The 6 exons are translated into a 263 amino acid protein with a 219 amino acid extracellular domain, a 23 amino acid transmembrane region, and a 21 amino acid intracellular domain.Tissue factor is also involved in thrombosis and inflammation associated with sepsis, atherosclerosis, tumor progression, and embryogenesis. It has been reported that humans can not survive without TF, and homozygous knockout of tissue factor in mouse embryos resulted in approximately 90% embryonic lethality by E10.5. Therefore, TF is essential for embryonic development but its specific functions are unknown.The study of zebrafish embryogenesis has yielded many insights into the development of the embryonic vasculature. Zebrafish embryos develop externally, are nearly transparent, and are thin enough for diffusion mediated oxygenation from the medium. In fact, zebrafish can survive the first week of development without a functional vasculature or heart beat, allowing a detailed analysis even in animals with severe cardiovascular defects. By contrast, avian and mammalian embryos die rapidly in the absence of a functional cardiovascular system.Recently, the zebrafish Tissue Factor gene (TF) has been cloned. Paralogs encode coagulation factorsⅢa andⅢb both show remarkable sequence identity to the human and mouse coagulation factor III gene. Here we turn to zebrafish to pursue the role of TF. We examined the role of coagulation factorⅢb (fib) during zebrafish embryonic development. Expression analysis revealed that endogenous fib was chronologically expressed in the pectoral fins and in the vicinity of the pharynx. Knockdown of fib by injection of a. f3b morpholino at the 1-2 cell stage caused distinct morphological defects in embryos, including edema in the fourth brain ventricle at early embryonic stages and occasional bleeding at later stages. Injection of TFb morpholino reduced expression of an oligodendrocyte marker gene (olig2) but had no effect on a neuronal marker gene (huc). Furthermore, f3b morphants displayed abnormal vascular patterning.In conclusion, zebrafish f3b is required for the development of the brain and vasculature during embryogenesis.

Related Dissertations

  1. Study on the Fine Structure of Intestine and Intestinal Mucosal Barrier in Zebrfish (Brachydanio Rerio),Q95-33
  2. The Joint Toxicity Evaluation of Chiral Organophosphrus Pesticides Enantiomers,X174
  3. The Study of Two Phenolic Organic Oxidated by Ozone and the Acute Toxicity,X703
  4. Induction of IGF Binding Protein-6 in Vascular Endothelial Cells is a Conserved Negative Feedback Mechanism in Hypoxia-induced Angiogenesis,Q46
  5. Craniofacial Abnormalities in Zebrafish Induced by Clomiphene Sodium Citrate,R114
  6. Expression of AQP4, TFPI2, CaM Protein in Different Stages of Human Cervical Lesions of Uygur,R737.33
  7. The Relationship between Tissue Factor and Acute Necrotizing Pancreatitis in Rats,R576
  8. Concentration Polarization of Atherogenic Lipids in Vessel in Zebrafish,R543.5
  9. Using Zebrafish as a Model to Study the Effect of Deguelin on COX/PGE2 Signaling Pathway,R285.5
  10. Evaluation of Cytotoxicity, Genotoxicity and Teratogenicity of Marine Sediments from Qingdao Coastal Areas Using Flounder Gill Cell Line FG and Zebrafish Embryos,X174
  11. Functional Analysis of PKB/Akt in Zebrafish Early Developmental Stages,Q55
  12. Original cadherin (Protocadherin) 18b in zebrafish embryos role in nervous system development,Q75
  13. Development and Evaluation of Time-resolved Fluorescence Immunoassay for Tissue Factor Pathway Inhibitor-2 (TFPI-2),R446.6
  14. Synthesis, DNA Binding Spectrscopy and Bio-toxicity on Zebrafish Embryo Studies of Cu (Ⅱ)/Zn (Ⅱ)-life Ligand Complexes,Q504
  15. Studies on the Toxicity of HgCl2 to Aquatic Organism,X503.2
  16. Maternal Transfer of Specific Antibodies (IgM) in Zebrafish Danio Rerio,Q5
  17. Behavioral Test of Fat Taste and the Cloning, Expression of Fat Taste Recepter in Fish,Q78
  18. Preliminary Study in the Negative Regulation of Wnt Pathway by Rspo3 in Zebrafish,Q75
  19. The Effects and Mechanisms of Monocrotophos Pesticide on Sex Differentiation of Zebrafish,X174
  20. Research of Plasma P-selectin and Tissue Factor Level in Lung Cancer Patients,R734.2
  21. Effect of 5-Aza-2-deoxycytidine on EC9706 Cell with Analysis of the Role of TFPI-2 Gene,R735.1

CLC: > Biological Sciences > Zoology > Animal morphology > Animal Embryology ( of animals,animal viviparous learn )
© 2012 www.DissertationTopic.Net  Mobile