Dissertation > Excellent graduate degree dissertation topics show

Study on the Preparation of Biosensor for Rhenol Detection

Author: YiLiLi
Tutor: LiTong
School: Hebei University of Science and Technology
Course: Environmental Science
Keywords: Biosensor Prussian blue film modified electrode Tyrosinase Glucose oxidase Silica sol - gel Phenolic compounds
CLC: X859
Type: Master's thesis
Year: 2009
Downloads: 163
Quote: 1
Read: Download Dissertation

Abstract


Phenolic compounds are highly toxic substances , have toxic effects on the human body and the environment , therefore, phenolic compounds detection is very important . In recent years , applied to measured phenol amperometric enzyme biosensor has high sensitivity , low cost , easy to realize the advantages of automation has attracted widespread attention . The main contents of the work are as follows: Prussian blue (PB) film modified glassy carbon electrode prepared with a \Prepared amperometric the tyrosinase measurement phenol biosensor new technology . Biosensor preparation conditions , operating conditions were optimized . Investigated tyrosinase measured phenol biosensor response performance of phenolic compounds , kinetic properties , selectivity , and stability . The results show that : tyrosinase measured phenol biosensor for phenol - response linear range of 0 ~ 100μmol / L, sensitivity to 27.25 mA / μmol · L -1 , the response time is less than 10s , seized a limit of 1.06μmol / L. Experimental proof of the biosensor to catechol and hydroquinone better response . Combination of silica sol - gel entrapped enzyme preparation amperometric based on Prussian blue of phenol measuring glucose oxidase electrode . Experimental results show: the prepared enzyme electrode , the optimum working potential of -0.15 V, the optimum phosphate buffer pH = 6.5 , and the most suitable temperature 35 ° C the best fixed amount of glucose oxidase 60μg, the best amount of glucose solution of 1 mmol / L. The effects of glucose oxidase electrode response properties of dynamical properties of the phenolic compounds , selectivity , and stability . The experimental results show that the phenol : glucose oxidase electrode showed good response and good selectivity . In addition , this work also examines the heavy metal the ion -Hg 2 < / sup > inhibition of tyrosinase biosensors . The experimental results show that : in the working potential of -0.20 V, the phosphate buffer at pH = 5.5 , of Hg the 2 the presence of inhibition of the current response of the biosensor of phenol , and the inhibition of the current size and Hg < sup> 2 linear relationship between the concentration in a certain range . The tyrosinase biosensors will be suppressed immersed in EDTA solution for 10 min , the biosensor can restore partial response performance .

Related Dissertations

  1. Study on Influential Factor to Methane Metabolic of Methane-utilizing Bacteria and Methoanol Detection Method,Q93
  2. Different electrical properties of carbon nanotubes covalently modified FAD molecular dynamics simulation,TB383.1
  3. Three parameters of human stress detection system,R318.0
  4. Rapid Detection of Zoonotic Pathogens Using Leaky Surface Acoustic Biosensor and Rolling Circle Amplification,R440
  5. Based graphene material , a Novel DNA biosensor,TP212.2
  6. Organic-inorganic hybrid materials and nanomaterials immobilized enzyme electrochemical biosensors,TP212.3
  7. High-level Expression of Cytochrome P450 in Prokaryotic System and Its Application in Biosensing for Persistent Organic Pollutants,Q78
  8. The Competitive Inhibition Assay and Experimental Study on Antibiotics and Melamine Residues in Milk,TS252.7
  9. Study on the Preparation and Properties of Functionalized Carbon Nanotubes,TB383.1
  10. Preparation of Gold-Maghemite Nanocomposites,TB383.1
  11. Basic Study of Photosensitive Functional Azopolymers as the Adsorption Materials of Protein,O631.3
  12. Screening of Cytotoxic Mangrove Endophytic Fungi and Study on Its Secondary Metabolites of Two Selected Strains,R284
  13. Research on Optofluidic Biosensor Based on Microcapillary,TP212.3
  14. Preliminary Study of Nitrifying Bacterial Sensor,X703
  15. Electrochemical Biosensors for Detection of Veterinary Drug and Pesticide Residues Based on Screen-printed Carbon Electrodes,TS207.53
  16. Improved Methods and Experiments for the Detection of Antibiotics Residues Based on Surface Plasmon Resonance Sensor,TS207.53
  17. The Research of Phenolic Compounds and Antioxidant Capacity in Brewing Barley and Malt,TS262.5
  18. Organic/Inorganic Conductive Nanocomposites Fabrication, Characterization and Application,TB383.1
  19. Study of the Application and the Direct Electrochemistry of Hemoglobin Immobilized on Nanoparticles Modified Glassy Carbon Electrodes,TB383.1
  20. Studies on Glucose Electrochemical Biosensor Based on Carbon Nanotubes and Platinum Nanoparticles,TB383.1
  21. Enzyme electrochemical sensors based on composites of graphene research,TP212.2

CLC: > Environmental science, safety science > Environmental Quality Assessment and Environmental Monitoring > Environmental monitoring equipment > Other monitoring equipment
© 2012 www.DissertationTopic.Net  Mobile